Kaposi's sarcoma-associated herpesvirus (human herpesvirus-8) ORF54 encodes a functional dUTPase expressed in the lytic replication cycle
E Kremmer, P Sommer, D Holzer, SA Galetsky, VA Molochkov, V Gurtsevitch, C Winkelmann, R Lisner, G Niedobitek and FA Grasser GSF-Institut fur Molekulare Immunologie, Marchioninistr. 25, 81377 Munchen, Germany The complete ORF54 of the Kaposi's sarcoma-associated herpesvirus (KSHV) (human herpes...
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Published in | Journal of general virology Vol. 80; no. 5; pp. 1305 - 1310 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
England
Soc General Microbiol
01.05.1999
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Subjects | |
Online Access | Get full text |
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Summary: | E Kremmer, P Sommer, D Holzer, SA Galetsky, VA Molochkov, V Gurtsevitch, C Winkelmann, R Lisner, G Niedobitek and FA Grasser
GSF-Institut fur Molekulare Immunologie, Marchioninistr. 25, 81377 Munchen, Germany
The complete ORF54 of the Kaposi's sarcoma-associated herpesvirus (KSHV)
(human herpesvirus-8; HHV-8) was cloned and expressed in E. coli. The
results show that KSHV/HHV-8 ORF54 encodes a functional dUTPase which
specifically hydrolyses dUTP to dUMP. Monoclonal antibodies against the
HHV-8 dUTPase detected a protein with the expected molecular mass of 35 kDa
in HHV-8-infected BCBL-1 cells. Induction of the lytic replication cycle of
HHV-8 by treatment of BCBL-1 cells with the phorbol ester TPA resulted in
an increased expression of the protein which was not inhibited by
phosphonoacetic acid, indicating that the protein is expressed early in the
lytic replication cycle. Moreover, the sporadic expression of the HHV-8
dUTPase in tissue sections of Kaposi's sarcoma was detected by
immunohistochemistry. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0022-1317 1465-2099 |
DOI: | 10.1099/0022-1317-80-5-1305 |