Lymphocytes as internal standard in oxidative burst analysis by cytometry: A new data analysis approach
We propose a new data analysis approach for reactive oxygen species detection using Dihydrorhodamine123 in blood monocytes and neutrophils. This approach, based on data transformation using lymphocytes as internal standard, allows to appreciate free radical production by monocytes also without Phorb...
Saved in:
| Published in | Journal of immunological methods Vol. 379; no. 1-2; pp. 61 - 65 |
|---|---|
| Main Authors | , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Netherlands
Elsevier B.V
31.05.2012
|
| Subjects | |
| Online Access | Get full text |
| ISSN | 0022-1759 1872-7905 1872-7905 |
| DOI | 10.1016/j.jim.2012.02.015 |
Cover
| Abstract | We propose a new data analysis approach for reactive oxygen species detection using Dihydrorhodamine123 in blood monocytes and neutrophils. This approach, based on data transformation using lymphocytes as internal standard, allows to appreciate free radical production by monocytes also without Phorbol 12-myristate 13-acetate (PMA) activation. In addition, this method is sensitive to differences in healthy subjects due to sub-pathological conditions, such as hypercholesterolemia. |
|---|---|
| AbstractList | We propose a new data analysis approach for reactive oxygen species detection using Dihydrorhodamine 123 in blood monocytes and neutrophils. This approach, based on data transformation using lymphocytes as internal standard, allows to appreciate free radical production by monocytes also without Phorbol 12-myristate 13-acetate (PMA) activation. In addition, this method is sensitive to differences in healthy subjects due to sub-pathological conditions, such as hypercholesterolemia.We propose a new data analysis approach for reactive oxygen species detection using Dihydrorhodamine 123 in blood monocytes and neutrophils. This approach, based on data transformation using lymphocytes as internal standard, allows to appreciate free radical production by monocytes also without Phorbol 12-myristate 13-acetate (PMA) activation. In addition, this method is sensitive to differences in healthy subjects due to sub-pathological conditions, such as hypercholesterolemia. We propose a new data analysis approach for reactive oxygen species detection using Dihydrorhodamine123 in blood monocytes and neutrophils. This approach, based on data transformation using lymphocytes as internal standard, allows to appreciate free radical production by monocytes also without Phorbol 12-myristate 13-acetate (PMA) activation. In addition, this method is sensitive to differences in healthy subjects due to sub-pathological conditions, such as hypercholesterolemia. We propose a new data analysis approach for reactive oxygen species detection using Dihydrorhodamine 123 in blood monocytes and neutrophils. This approach, based on data transformation using lymphocytes as internal standard, allows to appreciate free radical production by monocytes also without Phorbol 12-myristate 13-acetate (PMA) activation. In addition, this method is sensitive to differences in healthy subjects due to sub-pathological conditions, such as hypercholesterolemia. |
| Author | Riondino, Silvia Peluso, Ilaria Morabito, Giuseppa Serafini, Mauro La Farina, Francesca |
| Author_xml | – sequence: 1 givenname: Ilaria surname: Peluso fullname: Peluso, Ilaria email: peluso@inran.it organization: Istituto Nazionale di Ricerca per gli Alimenti e la Nutrizione (INRAN), Rome, Italy – sequence: 2 givenname: Giuseppa surname: Morabito fullname: Morabito, Giuseppa organization: Food and Nutrition Unit, IRCCS San Raffaele Pisana, Rome, Italy – sequence: 3 givenname: Silvia surname: Riondino fullname: Riondino, Silvia organization: Department of Laboratory Medicine and Advanced Biotechnologies, IRCCS San Raffaele Pisana, Rome, Italy – sequence: 4 givenname: Francesca surname: La Farina fullname: La Farina, Francesca organization: Department of Laboratory Medicine and Advanced Biotechnologies, IRCCS San Raffaele Pisana, Rome, Italy – sequence: 5 givenname: Mauro surname: Serafini fullname: Serafini, Mauro organization: Istituto Nazionale di Ricerca per gli Alimenti e la Nutrizione (INRAN), Rome, Italy |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/22405840$$D View this record in MEDLINE/PubMed |
| BookMark | eNqFkUFv1DAQhS1URLeFH8AFfOSSxePEdgKnqmoBaSUO0LPl2JPWqyRebG8h_x4v2wqJQ5FGGsn63vPovTNyMocZCXkNbA0M5PvteuunNWfA16wMiGdkBa3ileqYOCErxjivQInulJyltGWMAZPsBTnlvGGibdiK3G6WaXcX7JIxUZOonzPG2Yw0ZTM7E115oeGXdyb7e6T9PqZMTQGW5BPtF1qUYcIclw_0gs74kxbS_CXMbheDsXcvyfPBjAlfPexzcnN99f3yc7X5-unL5cWmsg2wXLW2d0oNMCCTUnArhTWy41LVyrqWD05wV3PnjGssWFDcmU45C0OLqhtcX5-Td0ff8u2PPaasJ58sjqOZMeyTBtlKxaGuxf_REp-QwJq6oG8e0H0_odO76CcTF_2YYwHUEbAxpBRx0NbnElmYczR-LF760Jje6tKYPjSmWRk4XAH_KB_Nn9K8PWoGE7S5jT7pm28FEKVgpeSfez4eCSxZ33uMOlmPs0XnI9qsXfBP-P8GHfy4Kw |
| CitedBy_id | crossref_primary_10_1016_j_jim_2013_02_005 crossref_primary_10_1002_cyto_a_22490 crossref_primary_10_1016_S1773_035X_18_30055_8 crossref_primary_10_1155_2016_5630985 crossref_primary_10_1016_j_fsi_2018_11_078 crossref_primary_10_1016_j_jim_2016_09_010 crossref_primary_10_1371_journal_pone_0068963 |
| Cites_doi | 10.1017/S0029665110001928 10.1177/0884533610385703 10.1161/ATVBAHA.107.147835 10.1111/j.1365-2249.1992.tb03072.x 10.1002/jlb.47.4.295 10.1189/jlb.0108023 10.1161/01.ATV.0000097769.47306.12 10.1016/S0009-8981(03)00086-X 10.1016/0022-1759(94)00247-T |
| ContentType | Journal Article |
| Copyright | 2012 Elsevier B.V. Copyright © 2012 Elsevier B.V. All rights reserved. |
| Copyright_xml | – notice: 2012 Elsevier B.V. – notice: Copyright © 2012 Elsevier B.V. All rights reserved. |
| DBID | FBQ AAYXX CITATION CGR CUY CVF ECM EIF NPM 7X8 7S9 L.6 |
| DOI | 10.1016/j.jim.2012.02.015 |
| DatabaseName | AGRIS CrossRef Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed MEDLINE - Academic AGRICOLA AGRICOLA - Academic |
| DatabaseTitle | CrossRef MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic AGRICOLA AGRICOLA - Academic |
| DatabaseTitleList | MEDLINE - Academic AGRICOLA MEDLINE |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database – sequence: 3 dbid: FBQ name: AGRIS url: http://www.fao.org/agris/Centre.asp?Menu_1ID=DB&Menu_2ID=DB1&Language=EN&Content=http://www.fao.org/agris/search?Language=EN sourceTypes: Publisher |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Medicine Biology |
| EISSN | 1872-7905 |
| EndPage | 65 |
| ExternalDocumentID | 22405840 10_1016_j_jim_2012_02_015 US201500177640 S0022175912000622 |
| Genre | Journal Article |
| GroupedDBID | --- --K --M .~1 0R~ 1B1 1RT 1~. 1~5 4.4 457 4G. 5GY 5RE 5VS 7-5 71M 8P~ 9JM AAAJQ AABNK AACTN AAEDT AAEDW AAIAV AAIKJ AAKOC AALRI AAOAW AAQFI AARKO AAXUO ABFNM ABFRF ABGSF ABJNI ABMAC ABUDA ABXDB ABYKQ ACDAQ ACGFO ACGFS ACIUM ACRLP ADBBV ADEZE ADMUD ADUVX AEBSH AEFWE AEHWI AEKER AENEX AFKWA AFTJW AFXIZ AGEKW AGHFR AGUBO AGYEJ AIEXJ AIKHN AITUG AJBFU AJOXV ALMA_UNASSIGNED_HOLDINGS AMFUW AMRAJ AXJTR BKOJK BLXMC CJTIS CNWQP CS3 DOVZS DU5 EBS EFJIC EFLBG EJD EO8 EO9 EP2 EP3 F5P FDB FIRID FNPLU FYGXN G-Q GBLVA HZ~ IH2 IHE J1W K-O KOM L7B LUGTX M41 MO0 N9A O-L O9- OAUVE OZT P-8 P-9 P2P PC. Q38 RIG ROL RPZ SDF SDG SDP SES SIN SPCBC SSI SSU SSZ T5K ZMT ~G- .55 .GJ 29K 3O- 53G AAQXK ABEFU ABPIF ABPTK AEQTP AFFNX AGRDE AHHHB AI. ASPBG AVWKF AZFZN D-I FBQ FEDTE FGOYB G-2 HMG HVGLF OHT R2- SEW UAP VH1 WUQ X7M XFK XPP Y6R ZGI AAHBH AATTM AAXKI AAYWO AAYXX ABWVN ACRPL ACVFH ADCNI ADNMO AEIPS AEUPX AFJKZ AFPUW AGCQF AGQPQ AGRNS AIGII AIIUN AKBMS AKRWK AKYEP ANKPU APXCP BNPGV CITATION SSH CGR CUY CVF ECM EFKBS EIF NPM 7X8 7S9 L.6 |
| ID | FETCH-LOGICAL-c410t-8cbd77f1fe06652c65ca6926737cd82fd52d32ddad4c1c172da97dc1f8e79fdb3 |
| IEDL.DBID | AIKHN |
| ISSN | 0022-1759 1872-7905 |
| IngestDate | Fri Sep 05 07:44:30 EDT 2025 Fri Sep 05 09:03:10 EDT 2025 Mon Jul 21 06:02:20 EDT 2025 Thu Apr 24 22:54:02 EDT 2025 Tue Jul 01 03:53:53 EDT 2025 Wed Dec 27 19:19:21 EST 2023 Fri Feb 23 02:31:07 EST 2024 |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 1-2 |
| Keywords | Macrophages Inflammation Cellular activation |
| Language | English |
| License | https://www.elsevier.com/tdm/userlicense/1.0 Copyright © 2012 Elsevier B.V. All rights reserved. |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c410t-8cbd77f1fe06652c65ca6926737cd82fd52d32ddad4c1c172da97dc1f8e79fdb3 |
| Notes | http://dx.doi.org/10.1016/j.jim.2012.02.015 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| PMID | 22405840 |
| PQID | 1002561043 |
| PQPubID | 23479 |
| PageCount | 5 |
| ParticipantIDs | proquest_miscellaneous_1686721335 proquest_miscellaneous_1002561043 pubmed_primary_22405840 crossref_citationtrail_10_1016_j_jim_2012_02_015 crossref_primary_10_1016_j_jim_2012_02_015 fao_agris_US201500177640 elsevier_sciencedirect_doi_10_1016_j_jim_2012_02_015 |
| ProviderPackageCode | CITATION AAYXX |
| PublicationCentury | 2000 |
| PublicationDate | 2012-05-31 |
| PublicationDateYYYYMMDD | 2012-05-31 |
| PublicationDate_xml | – month: 05 year: 2012 text: 2012-05-31 day: 31 |
| PublicationDecade | 2010 |
| PublicationPlace | Netherlands |
| PublicationPlace_xml | – name: Netherlands |
| PublicationTitle | Journal of immunological methods |
| PublicationTitleAlternate | J Immunol Methods |
| PublicationYear | 2012 |
| Publisher | Elsevier B.V |
| Publisher_xml | – name: Elsevier B.V |
| References | Noritake, Katsura, Shinomiya, Kanatani, Uwabe, Nagata, Tsuru (bb0030) 1992; 88 Wu, Meydani (bb0050) 2008; 84 Cathcart (bb0010) 2004; 24 Gustafson, Hammarstedt, Andersson, Smith (bb0020) 2007; 27 Kobzik, Godleski, Brain (bb0025) 1990; 47 Walrand, Valeix, Rodriguez, Ligot, Chassagne, Vasson (bb0040) 2003; 331 Galland (bb0015) 2010; 25 Wärnberg, Cunningham, Romeo, Marcos (bb0045) 2010; 69 Calder, Albers, Antoine, Blum, Bourdet-Sicard, Ferns, Folkerts, Friedmann, Frost, Guarner, Løvik, Macfarlane, Meyer, M'Rabet, Serafini, van Eden, van Loo, Vas Dias, Vidry, Winklhofer-Roob, Zhao (bb0005) 2009; 101 Vowells, Sekhsaria, Malech, Shalit, Fleisher (bb0035) 1995; 178 Galland (10.1016/j.jim.2012.02.015_bb0015) 2010; 25 Walrand (10.1016/j.jim.2012.02.015_bb0040) 2003; 331 Vowells (10.1016/j.jim.2012.02.015_bb0035) 1995; 178 Wärnberg (10.1016/j.jim.2012.02.015_bb0045) 2010; 69 Gustafson (10.1016/j.jim.2012.02.015_bb0020) 2007; 27 Cathcart (10.1016/j.jim.2012.02.015_bb0010) 2004; 24 Kobzik (10.1016/j.jim.2012.02.015_bb0025) 1990; 47 Calder (10.1016/j.jim.2012.02.015_bb0005) 2009; 101 Wu (10.1016/j.jim.2012.02.015_bb0050) 2008; 84 Noritake (10.1016/j.jim.2012.02.015_bb0030) 1992; 88 |
| References_xml | – volume: 27 start-page: 2276 year: 2007 end-page: 2283 ident: bb0020 article-title: Inflamed adipose tissue: a culprit underlying the metabolic syndrome and atherosclerosis publication-title: Arterioscler. Thromb. Vasc. Biol. – volume: 69 start-page: 400 year: 2010 end-page: 406 ident: bb0045 article-title: Physical activity, exercise and low-grade systemic inflammation publication-title: Proc. Nutr. Soc. – volume: 84 start-page: 900 year: 2008 end-page: 914 ident: bb0050 article-title: Age-associated changes in immune and inflammatory responses: impact of vitamin E intervention publication-title: J. Leukoc. Biol. – volume: 101 start-page: S1 year: 2009 end-page: S45 ident: bb0005 article-title: Inflammatory disease processes and interactions with nutrition publication-title: Br. J. Nutr. – volume: 25 start-page: 634 year: 2010 end-page: 640 ident: bb0015 article-title: Diet and inflammation publication-title: Nutr. Clin. Pract. – volume: 88 start-page: 269 year: 1992 end-page: 274 ident: bb0030 article-title: Intracellular hydrogen peroxide production by peripheral phagocytes from diabetic patients. Dissociation between polymorphonuclear leucocytes and monocytes publication-title: Clin. Exp. Immunol. – volume: 178 start-page: 89 year: 1995 end-page: 97 ident: bb0035 article-title: Flow cytometric analysis of the granulocyte respiratory burst: a comparison study of fluorescent probes publication-title: J. Immunol. Methods – volume: 47 start-page: 295 year: 1990 end-page: 303 ident: bb0025 article-title: Oxidative metabolism in the alveolar macrophage: analysis by flow cytometry publication-title: J. Leukoc. Biol. – volume: 24 start-page: 23 year: 2004 end-page: 28 ident: bb0010 article-title: Regulation of superoxide anion production by NADPH oxidase in monocytes/macrophages: contributions to atherosclerosis publication-title: Arterioscler. Thromb. Vasc. Biol. – volume: 331 start-page: 103 year: 2003 end-page: 110 ident: bb0040 article-title: Flow cytometry study of polymorphonuclear neutrophil oxidative burst: a comparison of three fluorescent probes publication-title: Clin. Chim. Acta – volume: 69 start-page: 400 issue: 3 year: 2010 ident: 10.1016/j.jim.2012.02.015_bb0045 article-title: Physical activity, exercise and low-grade systemic inflammation publication-title: Proc. Nutr. Soc. doi: 10.1017/S0029665110001928 – volume: 25 start-page: 634 issue: 6 year: 2010 ident: 10.1016/j.jim.2012.02.015_bb0015 article-title: Diet and inflammation publication-title: Nutr. Clin. Pract. doi: 10.1177/0884533610385703 – volume: 27 start-page: 2276 issue: 11 year: 2007 ident: 10.1016/j.jim.2012.02.015_bb0020 article-title: Inflamed adipose tissue: a culprit underlying the metabolic syndrome and atherosclerosis publication-title: Arterioscler. Thromb. Vasc. Biol. doi: 10.1161/ATVBAHA.107.147835 – volume: 88 start-page: 269 issue: 2 year: 1992 ident: 10.1016/j.jim.2012.02.015_bb0030 article-title: Intracellular hydrogen peroxide production by peripheral phagocytes from diabetic patients. Dissociation between polymorphonuclear leucocytes and monocytes publication-title: Clin. Exp. Immunol. doi: 10.1111/j.1365-2249.1992.tb03072.x – volume: 47 start-page: 295 issue: 4 year: 1990 ident: 10.1016/j.jim.2012.02.015_bb0025 article-title: Oxidative metabolism in the alveolar macrophage: analysis by flow cytometry publication-title: J. Leukoc. Biol. doi: 10.1002/jlb.47.4.295 – volume: 84 start-page: 900 issue: 4 year: 2008 ident: 10.1016/j.jim.2012.02.015_bb0050 article-title: Age-associated changes in immune and inflammatory responses: impact of vitamin E intervention publication-title: J. Leukoc. Biol. doi: 10.1189/jlb.0108023 – volume: 101 start-page: S1 issue: Suppl. 1 year: 2009 ident: 10.1016/j.jim.2012.02.015_bb0005 article-title: Inflammatory disease processes and interactions with nutrition publication-title: Br. J. Nutr. – volume: 24 start-page: 23 issue: 1 year: 2004 ident: 10.1016/j.jim.2012.02.015_bb0010 article-title: Regulation of superoxide anion production by NADPH oxidase in monocytes/macrophages: contributions to atherosclerosis publication-title: Arterioscler. Thromb. Vasc. Biol. doi: 10.1161/01.ATV.0000097769.47306.12 – volume: 331 start-page: 103 issue: 1–2 year: 2003 ident: 10.1016/j.jim.2012.02.015_bb0040 article-title: Flow cytometry study of polymorphonuclear neutrophil oxidative burst: a comparison of three fluorescent probes publication-title: Clin. Chim. Acta doi: 10.1016/S0009-8981(03)00086-X – volume: 178 start-page: 89 year: 1995 ident: 10.1016/j.jim.2012.02.015_bb0035 article-title: Flow cytometric analysis of the granulocyte respiratory burst: a comparison study of fluorescent probes publication-title: J. Immunol. Methods doi: 10.1016/0022-1759(94)00247-T |
| SSID | ssj0001060 |
| Score | 2.0502746 |
| Snippet | We propose a new data analysis approach for reactive oxygen species detection using Dihydrorhodamine123 in blood monocytes and neutrophils. This approach,... We propose a new data analysis approach for reactive oxygen species detection using Dihydrorhodamine 123 in blood monocytes and neutrophils. This approach,... |
| SourceID | proquest pubmed crossref fao elsevier |
| SourceType | Aggregation Database Index Database Enrichment Source Publisher |
| StartPage | 61 |
| SubjectTerms | Adult Cellular activation Female Flow Cytometry Humans hypercholesterolemia Inflammation lymphocytes Lymphocytes - metabolism Macrophages Male Middle Aged monocytes Monocytes - metabolism neutrophils Neutrophils - metabolism reactive oxygen species Reactive Oxygen Species - metabolism Respiratory Burst Statistics as Topic - methods Tetradecanoylphorbol Acetate - pharmacology |
| Title | Lymphocytes as internal standard in oxidative burst analysis by cytometry: A new data analysis approach |
| URI | https://dx.doi.org/10.1016/j.jim.2012.02.015 https://www.ncbi.nlm.nih.gov/pubmed/22405840 https://www.proquest.com/docview/1002561043 https://www.proquest.com/docview/1686721335 |
| Volume | 379 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1La9wwEBbJhpZeQpu2SfoIKvRUcCPLsmXntoSE7SO5tAu5Cb0cHNJ1yDoQX_LbO2NLWwrNHgq6WGiwrJFHn-ZJyEdmsZRVliW1Lkq4oBiZGMtsYqWomHAs9w71HWfnxWwuvl7kFxvkOMbCoFtlkP2jTB-kdeg5DKt5eNM0GOPLAU_nVTpEm3CQw1s8q4p8QramX77NzlcCGW49LCYNR4Jo3BzcvK4ajEdHjSA0LI777-Nps9bt4yB0OIxOn5PtgCLpdJzoC7LhFzvkyVhXst8hT8-CxfwlufzeA79a2wOkpHpJm1EDeE2jDgF6aHvfuCEBOIUlXnZUh0wl1PQUKNtfvrvtj-iUAgan6FP6Z0TMSf6KzE9Pfh7PklBcIbEiZV1SWuOkrNPao_GF2yK3uqg4lq2xruS1y7nLuHPaCZtagDlOV9LZtC69rGpnstdksmgXfo9Qw0VptDS1KLzwQhrttbRp5o2wBiDdPmFxTZUNmcexAMa1ii5mVwrYoJANikFLgeTTiuRmTLuxbrCIjFJ_7R0Fx8I6sj1gqtKXIE3V_AdH3Q_IJ1kItk8-RE4r-N3QhqIXvr1bYopnjpBTZGvGFGUBF-ssg1fsjttk9RGIoADzsTf_N-e35Bk-je4L78iku73z7wEVdeaAbH5-SA_C3v8N3iQJfA |
| linkProvider | Elsevier |
| linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3dT9UwFG8Qo_JCFEVQ1Jr4ZDLp2m7dfCMEctV7eZGb8Nb0a2QE7wh3JO6Fv91zthVigvfBpE_dadb1dKe_nk9CPjGHpayESCqTF3BBsSqxjrnEKVky6VkWPOo7Zif5ZC6_n2Vna-QwxsKgW-Uo-weZ3kvrsWd_XM39q7rGGF8OeDor0z7ahIMcfiwzodCv78vtvZ8H3HlYTBmO5NG02Tt5XdQYjY76QGhYGvfhw-lRZZp_Q9D-KDp-TjZHDEkPhmm-IGthsUWeDFUluy3ydDbay1-S82kH3GpcB4CSmiWtB_3fJY0aBOihze_a9-m_KSzwsqVmzFNCbUdhZPMrtNfdV3pAAYFT9Ci9p4gZyV-R-fHR6eEkGUsrJE6mrE0KZ71SVVoFNL1wl2fO5CXHojXOF7zyGfeCe2-8dKkDkONNqbxLqyKosvJWbJP1RbMIO4RaLgtrlK1kHmSQyppglEtFsNJZAHS7hMU11W7MO47lLy51dDC70MAGjWzQDFoKQz7fDbkakm6sIpaRUfqvnaPhUFg1bAeYqs05yFI9_8lR8wPSSeWS7ZKPkdMafja0oJhFaG6WmOCZI-CUYgVNXuRwrRYCXvF62CZ3H4H4CRAfe_N_c_5Ank1OZ1M9_Xby4y3ZwCeDI8MeWW-vb8I7wEetfd_v_z8cDApH |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Lymphocytes+as+internal+standard+in+oxidative+burst+analysis+by+cytometry%3A+A+new+data+analysis+approach&rft.jtitle=Journal+of+immunological+methods&rft.au=Peluso%2C+Ilaria&rft.au=Morabito%2C+Giuseppa&rft.au=Riondino%2C+Silvia&rft.au=La+Farina%2C+Francesca&rft.date=2012-05-31&rft.pub=Elsevier+B.V&rft.issn=0022-1759&rft.eissn=1872-7905&rft.volume=379&rft.issue=1-2&rft.spage=61&rft.epage=65&rft_id=info:doi/10.1016%2Fj.jim.2012.02.015&rft.externalDocID=S0022175912000622 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0022-1759&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0022-1759&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0022-1759&client=summon |