Lymphocytes as internal standard in oxidative burst analysis by cytometry: A new data analysis approach

We propose a new data analysis approach for reactive oxygen species detection using Dihydrorhodamine123 in blood monocytes and neutrophils. This approach, based on data transformation using lymphocytes as internal standard, allows to appreciate free radical production by monocytes also without Phorb...

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Bibliographic Details
Published inJournal of immunological methods Vol. 379; no. 1-2; pp. 61 - 65
Main Authors Peluso, Ilaria, Morabito, Giuseppa, Riondino, Silvia, La Farina, Francesca, Serafini, Mauro
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 31.05.2012
Subjects
Adult
Cellular activation
Female
Flow Cytometry
Humans
hypercholesterolemia
Inflammation
lymphocytes
Lymphocytes - metabolism
Macrophages
Male
Middle Aged
monocytes
Monocytes - metabolism
neutrophils
Neutrophils - metabolism
reactive oxygen species
Reactive Oxygen Species - metabolism
Respiratory Burst
Statistics as Topic - methods
Tetradecanoylphorbol Acetate - pharmacology
Macrophages
Inflammation
Cellular activation
Online AccessGet full text
ISSN0022-1759
1872-7905
1872-7905
DOI10.1016/j.jim.2012.02.015

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Summary:We propose a new data analysis approach for reactive oxygen species detection using Dihydrorhodamine123 in blood monocytes and neutrophils. This approach, based on data transformation using lymphocytes as internal standard, allows to appreciate free radical production by monocytes also without Phorbol 12-myristate 13-acetate (PMA) activation. In addition, this method is sensitive to differences in healthy subjects due to sub-pathological conditions, such as hypercholesterolemia.
Bibliography:http://dx.doi.org/10.1016/j.jim.2012.02.015
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ISSN:0022-1759
1872-7905
1872-7905
DOI:10.1016/j.jim.2012.02.015