Enhancement of transdermal permeation of phycobiliprotein by nanodispersion with lipopeptide from Bacillus subtilis

• Published in: Biotechnology and bioprocess engineering Vol. 30; no. 2; pp. 401 - 410
• Main Authors: Lee, Byung Man, Jang, Bum Jun, Shin, Jae Ahn, Shin, Hwa Sung
• Format: Journal Article
• Language: English
• Published: Seoul The Korean Society for Biotechnology and Bioengineering 01.04.2025; Springer Nature B.V; 한국생물공학회
• Subjects: antioxidants; Bacillus subtilis; Biocompatibility; Biomedical materials; biosurfactants; Biotechnology; Chemistry; Chemistry and Materials Science; Cosmetics; Cytotoxicity; Drug delivery; drugs; emulsifiers; Ethanol; Fermentation; In vitro methods and tests; Industrial and Production Engineering; inflammation; lipopeptides; Micelles; Nanoparticles; permeability; Permeation; photosynthesis; Photosynthetic pigments; phycobiliprotein; Research Paper; Skin; Tissue engineering; transdermal application; 생물공학; Biosurfactant; Transdermal delivery; Phycobiliprotein; Permeation enhancer; Lipopeptide
• ISSN: 1226-8372; 1976-3816
• DOI: 10.1007/s12257-024-00166-7

Lipopeptide is used as an emulsifier to stably formulate bioactives-containing nanoparticles as a biosurfactant. Phycobilprotein (PBP) is a photosynthetic pigment with various physiological activities and is actively used for cosmetics or pharmaceuticals. This study aims to increase the transdermal delivery of PBP by forming a PBP nanodispersion in water using lipopeptide derived from Bacillus subtilis . Lipopeptide produced through B. subtilis fermentation, acid precipitation, and ethanol extraction showed low cytotoxicity and inflammation and demonstrated moisturizing and antioxidant abilities in in vitro tests. Additionally, treatment to PBP resulted in obtaining a nanodispersion depending on lipopeptide critical micelle concentration, and experimental results on 3D tissue-engineered or skin substitutes showed increased permeation compared to free PBP. This study is significant in that it demonstrates the role of lipopeptide derived from GRAS B. subtilis as a skin permeation enhancer for PBP, ensuring safety and enhancing the applicability of PBP as a cosmetic or biomedical material by improving its skin penetration.