The complete nucleotide sequence of the Atlantic salmon growth hormone I gene

• Published in: Biochimica et biophysica acta Vol. 1130; no. 3; pp. 345 - 348
• Main Authors: Male, Rune, H. Nerland, Audun, Lorens, James B., Telle, Wenche, Lossius, Ivar, Totland, Geir K.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 06.04.1992; Elsevier
• Subjects: Amino Acid Sequence; Animals; Atlantic salmon; Base Sequence; Biological and medical sciences; Blotting, Southern; Cloning, Molecular; ddbj/x61938; embl/x61938; Fish Proteins; Fundamental and applied biological sciences. Psychology; genbank/x61938; Genes. Genome; Genomic sequence; Growth hormone; Growth Hormone - chemistry; Growth Hormone - genetics; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; nucleotide sequences; Repetitive Sequences, Nucleic Acid - genetics; Restriction Mapping; Salmo salar; Salmon - genetics; Sequence Alignment; somatotropin; structural genes; Growth hormone; Atlantic salmon; Genomic sequence; Protein hormone; Vertebrata; Nucleotide sequence; Pisces; STH; Salmo salar; Restriction map; Gene organization
• ISSN: 0167-4781; 0006-3002; 1879-2634; 1878-2434
• DOI: 10.1016/0167-4781(92)90452-6

Two closely related genes encoding growth hormone were isolated from Atlantic salmon by genomic cloning. From one of these genes a total of 6500 nucleotides were determined including 3900 nucleotides in exons and introns and about 600 and 2000 nucleotides in 5′ and 3′ flanking regions. The gene is organized in six exons and encodes a polypeptide of 210 amino acids including a 22 amino acids signal sequence. The promoter region contains a typical TATA box 21 nucleotides upstream from the transcription start site. At the 3′ end, three putative poly(A) signal sequences are present. The last two are within a 121 nt inverted repeat.