HPTLC methods to assay active ingredients in pharmaceutical formulations: A review of the method development and validation steps

High-performance thin-layer chromatography (HPTLC) is still increasingly finding its way in pharmaceutical analysis in some parts of the world. With the advancements in the stationary phases and the introduction of densitometers as detection equipment, the technique achieves for given applications a...

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Bibliographic Details
Published inJournal of pharmaceutical and biomedical analysis Vol. 66; pp. 11 - 23
Main Authors Shewiyo, D.H., Kaale, E., Risha, P.G., Dejaegher, B., Smeyers-Verbeke, J., Heyden, Y. Vander
Format Journal Article
LanguageEnglish
Published Amsterdam Elsevier B.V 01.07.2012
Elsevier
Subjects
Active ingredient
active ingredients
Analysis
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Chromatography, High Pressure Liquid - methods
Chromatography, Thin Layer - methods
densitometers
Densitometry
drug formulations
Fundamental and applied biological sciences. Psychology
General pharmacology
high performance liquid chromatography
HPTLC
Humans
Medical sciences
Method development
Method validation
Pharmaceutical analysis
Pharmaceutical formulation
Pharmaceutical Preparations - analysis
Pharmaceutical Preparations - chemistry
Pharmacology. Drug treatments
thin layer chromatography
Validation Studies as Topic
Active ingredient
Pharmaceutical formulation
Pharmaceutical analysis
Method development
Method validation
HPTLC
Drug
Validation
High performance thin layer chromatography
Chemical analysis
Review
Active pharmaceutical ingredient
Formulation
Development
Dosage form
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Summary:High-performance thin-layer chromatography (HPTLC) is still increasingly finding its way in pharmaceutical analysis in some parts of the world. With the advancements in the stationary phases and the introduction of densitometers as detection equipment, the technique achieves for given applications a precision and trueness comparable to high-performance liquid chromatography (HPLC). In this review, the literature is surveyed for developed and validated HPTLC methods to assay active ingredients in pharmaceutical formulations published in the period 2005–2011. Procedures and approaches for method development, validation and quantitative assays are compared with the standard ways of conducting them. Applications of HPTLC in some other areas are also briefly highlighted.
Bibliography:http://dx.doi.org/10.1016/j.jpba.2012.03.034
ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-3
content type line 23
ObjectType-Review-1
ISSN:0731-7085
1873-264X
DOI:10.1016/j.jpba.2012.03.034