Characterization of phenolic compounds and antinociceptive activity of Sempervivum tectorum L. leaf juice

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 70; pp. 143 - 150
• Main Authors: Alberti, Ágnes, Béni, Szabolcs, Lackó, Erzsébet, Riba, Pál, Al-Khrasani, Mahmoud, Kéry, Ágnes
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.11.2012; Elsevier
• Subjects: Acetic Acid; acids; Analgesics - chemistry; Analgesics - isolation & purification; Analgesics - pharmacology; Analysis; Analytical, structural and metabolic biochemistry; Animals; Behavior, Animal - drug effects; Biological and medical sciences; Calibration; Chromatography, High Pressure Liquid - standards; Crassulaceae; Crassulaceae - chemistry; Disease Models, Animal; Flavonol; Freeze Drying; Fundamental and applied biological sciences. Psychology; General pharmacology; glycosides; Glycosides - pharmacology; high performance liquid chromatography; Houseleek; ionization; juices; kaempferol; Kaempferols - pharmacology; LC–MS; leaves; Magnetic Resonance Spectroscopy - standards; Male; Medical sciences; Mice; Molecular Structure; nuclear magnetic resonance spectroscopy; pain; Pain - chemically induced; Pain - prevention & control; Pharmacology. Drug treatments; Phenolic acid; phenolic compounds; Phenols - chemistry; Phenols - isolation & purification; Phenols - pharmacology; Plant Extracts - chemistry; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; Plant Leaves; quantitative analysis; Reference Standards; Reproducibility of Results; Sempervivum tectorum; Sempervivum tectorum L; Spectrometry, Mass, Electrospray Ionization - standards; tandem mass spectrometry; Tandem Mass Spectrometry - standards; Europe; Sempervivum tectorum L; Flavonol; Phenolic acid; Houseleek; Crassulaceae; LC–MS; Pharmacognosy; HPLC chromatography; Plant leaf; Flavonoid; Medicinal plant; Characterization; Polyphenol; LC-MS; Analgesic; Folk medicine; Dicotyledones; Angiospermae; Plant origin; Phenols; Spermatophyta; Mass spectrometry
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/j.jpba.2012.06.017

Sempervivum tectorum L. (houseleek) leaf juice has been known as a traditional herbal remedy. The aim of the present study was the chemical characterization of its phenolic compounds and to develop quantitation methods for its main flavonol glycoside, as well as to evaluate its antinociceptive activity. Lyophilized houseleek leaf juice was studied by HPLC–DAD coupled to electrospray ionization tandem mass spectrometry (LC–ESI-MS/MS) to identify flavonol glycosides, hydroxy-benzoic and hydroxy-cinnamic acids. Ten flavonol glycosides and sixteen phenolic acid compounds were identified or tentatively characterized. Structure of the main flavonol compound was identified by nuclear magnetic resonance spectroscopy. Three characteristic kaempferol glycosides were isolated and determined by LC–ESI-MS/MS with external calibration method, using the isolated compounds as standard. The main flavonol glycoside was also determined by HPLC–DAD. Validated HPLC–DAD and LC–ESI-MS/MS methods were developed to quantify kaempferol-3-O-rhamnosyl-glucoside-7-O-rhamnoside and two other kaempferol glycosides. Antinociceptive activity of houseleek leaf juice was investigated by writhing test of mice. Sempervivum extract significantly reduced pain in the mouse writhing test.