Structure of Drosophila virilis glycerol-3-phosphate dehydrogenase gene and a comparison with the Drosophila melanogaster gene

• Published in: Biochimica et biophysica acta Vol. 1131; no. 2; pp. 233 - 238
• Main Authors: Tominaga, Hiroko, Shiba, Tadayoshi, Narise, Sumiko
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 15.06.1992; Elsevier
• Subjects: amino acid sequences; Animals; Base Sequence; Biological and medical sciences; D. melanogaster; D. virilis; ddbj/d01107; DNA; DNA sequence; Drosophila - enzymology; Drosophila - genetics; Drosophila melanogaster; Drosophila melanogaster - enzymology; Drosophila melanogaster - genetics; Drosophila virilis; Fundamental and applied biological sciences. Psychology; genes; Genes. Genome; glycerol-3-phosphate dehydrogenase; Glycerol-3-phosphate dehydrogenase gene; Glycerolphosphate Dehydrogenase - genetics; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; nucleotide sequences; Restriction Mapping; DNA sequence; D. virilis; Glycerol-3-phosphate dehydrogenase gene; D. melanogaster; Glycerol-3-phosphate dehydrogenase (NAD+); Nucleotide sequence; Enzyme; Isozyme; Insecta; Interspecific comparison; Homology; Gene organization; Drosophila virilis; Drosophilidae; Gene; Arthropoda; Invertebrata; Drosophila melanogaster; Diptera
• ISSN: 0167-4781; 0006-3002; 1879-2634; 1878-2434
• DOI: 10.1016/0167-4781(92)90086-F

The complete glycerol-3-phosphate dehydrogenase gene of Drosophila virilis isolated by screening with αGPDH M cDNA of the adult fly was sequenced. The gene contains eight exons spread over a total of approximate 8 kb DNA. Its exon/intron organization is identical to that of D. melanogaster. A single transcription initiation site was determined by primer extension. The stop codons are located at the 3′ end of each of the exons 6 to 8. TATA and CAAT boxes are present upstream of the transcriptional start site. Adult αGPDH protein is encoded by exons 1 to 6 and exon 8. Comparison of the sequence with that of D. melanogaster showed that the homology of the nucleotide sequence of the coding region is 85% and that the homology of the amino acid sequence is 98%. On the contrary, the non-coding region is quite different in length and nucleotide sequence.