Gluconeogenesis in goat hepatocytes is affected by calcium, ammonia and other key metabolites but not primarily through cytosolic redox state

• Published in: Comparative biochemistry and physiology. B, Comparative biochemistry Vol. 88; no. 1; pp. 193 - 201
• Main Authors: Aiello, R.J., Armentano, L.E.
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 1987; Elsevier Science
• Subjects: Aminooxyacetic Acid - pharmacology; ammonia; Ammonium Chloride - pharmacology; Animals; Biological and medical sciences; calcium; Calcium - pharmacology; Cytosol - metabolism; Ethanol - pharmacology; Fundamental and applied biological sciences. Psychology; gluconeogenesis; Gluconeogenesis - drug effects; goats; Goats - metabolism; lactates; Lactates - metabolism; Lactic Acid; liver; Liver - drug effects; Liver - metabolism; Liver. Bile. Biliary tracts; metabolites; Oxidation-Reduction; propionates; Propionates - metabolism; Quinolinic Acid; Quinolinic Acids - pharmacology; Vertebrates: digestive system; Calcium; Ruminant animal; Metabolite; Liver; Glucose; Metabolism; Ammonia; Vertebrata; Mammalia; Hepatocyte; Goat; Artiodactyla; Gluconeogénesis; Ungulata
• ISSN: 0305-0491
• DOI: 10.1016/0305-0491(87)90100-3

1. 1. Gluconeogenesis from propionate and lactate was studied in caprine hepatocytes. 2. 2. Reducing cytosol with additions of ETOH, ammonium, or lactate decreased [2- 14C]propionate conversion to glucose. 3. 3. Calcium oxidized the cytosol and increased gluconeogenesis from propionate by 198% and from lactate by 220%. 4. 4. Cells isolated from lactating does and wethers differed quantitatively in propionate conversion to glucose and response to calcium. 5. 5. Acetoacetate decreased and 3-OH-butyrate slightly increased glucose production from propionate. 6. 6. Neither ketone body had any significant effect on gluconeogenesis from lactate. 7. 7. Results reported herein suggest gluconeogenesis from propionate is not limited by lack of cytosolic reducing equivalents.