Cadmium Highlights Common and Specific Responses of Two Freshwater Sentinel Species, Dreissena polymorpha and Dreissena rostriformis bugensis

• Published in: Proteomes Vol. 12; no. 2; p. 10
• Main Authors: Bultelle, Florence, Le Saux, Aimie, David, Elise, Tanguy, Arnaud, Devin, Simon, Olivier, Stéphanie, Poret, Agnès, Chan, Philippe, Louis, Fanny, Delahaut, Laurence, Pain-Devin, Sandrine, Péden, Romain, Vaudry, David, Le Foll, Frank, Rocher, Béatrice
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 26.03.2024; MDPI
• Subjects: Bioaccumulation; Biomarkers; Biomonitoring; Cadmium; Calcium signalling; Cell death; Cellular stress response; Consortia; Controlled conditions; Cytoskeleton; DNA damage; Dreissena polymorpha; Dreissena rostriformis bugensis; Endocytosis; Environmental Sciences; Exocytosis; Glutathione; Heavy metals; Life Sciences; Mass spectroscopy; Membrane trafficking; Metal ions; Mollusks; Pollutants; proteoforms; Proteolysis; Proteomes; Proteomics; quagga mussel; Respiration; Scientific imaging; Sibling species; stress; Temperature; Water; zebra mussel; France; North America; Western Europe; quagga mussel; stress; proteoforms; biomonitoring; zebra mussel; quagga mussel zebra mussel proteoforms stress biomonitoring; Proteomic Analysis
• ISSN: 2227-7382; 2227-7382
• DOI: 10.3390/proteomes12020010

Zebra mussel (ZM), , commonly used as a sentinel species in freshwater biomonitoring, is now in competition for habitat with quagga mussel (QM), This raises the question of the quagga mussel's use in environmental survey. To better characterise QM response to stress compared with ZM, both species were exposed to cadmium (100 µg·L ), a classic pollutant, for 7 days under controlled conditions. The gill proteomes were analysed using two-dimensional electrophoresis coupled with mass spectrometry. For ZM, 81 out of 88 proteoforms of variable abundance were identified using mass spectrometry, and for QM, 105 out of 134. Interestingly, the proteomic response amplitude varied drastically, with 5.6% of proteoforms of variable abundance (DAPs) in ZM versus 9.4% in QM. QM also exhibited greater cadmium accumulation. Only 12 common DAPs were observed. Several short proteoforms were detected, suggesting proteolysis. Functional analysis is consistent with the pleiotropic effects of the toxic metal ion cadmium, with alterations in sulphur and glutathione metabolisms, cellular calcium signalling, cytoskeletal dynamics, energy production, chaperone activation, and membrane events with numerous proteins involved in trafficking and endocytosis/exocytosis processes. Beyond common responses, the sister species display distinct reactions, with cellular response to stress being the main category involved in ZM as opposed to calcium and cytoskeleton alterations in QM. Moreover, QM exhibited greater evidence of proteolysis and cell death. Overall, these results suggest that QM has a weaker stress response capacity than ZM.