Effect of Aluminium on Lipid Peroxidation of Human High Density Lipoproteins

• Published in: Free radical research Vol. 37; no. 5; pp. 515 - 521
• Main Authors: Ferretti, G., Marchionni, C., Bacchetti, T., Galeazzi, T., Dousset, N.
• Format: Journal Article
• Language: English
• Published: England Informa UK Ltd 01.05.2003; Taylor & Francis
• Subjects: Aluminium; Aluminum - pharmacology; Diphenylhexatriene - analogs & derivatives; Diphenylhexatriene - chemistry; Dose-Response Relationship, Drug; Ferrous Compounds - pharmacology; Fluorescence Polarization; Fluorescent Dyes - chemistry; High Density Lipoproteins; Humans; Hydrogen-Ion Concentration; Iron; Lipid Peroxidation; Lipid Peroxidation - drug effects; Lipid Peroxides - metabolism; Lipoproteins, HDL - blood; Lipoproteins, HDL - metabolism; Neurological Diseases; Oxidation-Reduction - drug effects; Physico-chemical Properties; Spectrometry, Fluorescence; Thiobarbituric Acid Reactive Substances - chemistry; Thiobarbituric Acid Reactive Substances - metabolism; Tryptophan - chemistry
• ISSN: 1071-5762; 1029-2470
• DOI: 10.1080/1071576031000083125

We investigated the effect of aluminium (Al 3+ ) on lipid peroxidation and physico-chemical properties of high density lipoproteins (HDL) isolated from human plasma. Our results demonstrated that Al 3+ enhances lipid peroxidation of human HDL as shown by the significant increase in lipid hydroperoxides in Al-treated HDL with respect to control HDL. The oxidative effect was higher at acid pH (pH 5.5) with respect to pH 7.4. Moreover, a stimulating effect of Al 3+ on iron-induced lipid peroxidation of HDL was demonstrated. The study of the effect of Al 3+ on the physico-chemical properties of HDL, using the fluorescence polarization (Pf) of the probes TMA-DPH (1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene iodide) and DPH (1,6-diphenyl-1,3,5-hexatriene), showed a significant decrease of Pf in Al-treated HDL with respect to control. These results suggest that Al 3+ induces a decrease of molecular order at the lipoprotein surface. Moreover, the study of tryptophan (Trp) fluorescence demonstrated that aluminium induces structural modifications of HDL apoproteins and on HDL physico-chemical properties. The effect of Al 3+ on lipid peroxidation of HDL was observed at aluminium concentrations similar to those observed in the brain of patients affected by neurological diseases. Aluminium-induced oxidative damage of HDL could be involved in the development of neurological diseases.