Imaging and differentiation of mouse embryo tissues by ToF-SIMS

Time-of-flight secondary ion mass spectrometry (ToF-SIMS) equipped with a gold ion gun was used to image mouse embryo sections and differentiate tissue types (brain, spinal cord, skull, rib, heart and liver). Embryos were paraffin-embedded and then deparaffinized. The robustness and repeatability of...

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Bibliographic Details
Published inInternational journal of mass spectrometry Vol. 260; no. 2; pp. 137 - 145
Main Authors Wu, Ligang, Lu, Xiaochen, Kulp, Kristen S., Knize, Mark G., Berman, Elena S.F., Nelson, Erik J., Felton, James S., Wu, Kuang Jen J.
Format Journal Article
LanguageEnglish
Published Elsevier B.V 01.02.2007
Subjects
Image PCA
Mouse embryo
Paraffin-embedded
ToF-SIMS
Paraffin-embedded
Image PCA
ToF-SIMS
Mouse embryo
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Summary:Time-of-flight secondary ion mass spectrometry (ToF-SIMS) equipped with a gold ion gun was used to image mouse embryo sections and differentiate tissue types (brain, spinal cord, skull, rib, heart and liver). Embryos were paraffin-embedded and then deparaffinized. The robustness and repeatability of the method was determined by analyzing ten tissue slices from three different embryos over a period of several weeks. Using principal component analysis (PCA) to reduce the spectral data generated by ToF-SIMS, histopathologically identified tissue types of the mouse embryos can be differentiated based on the characteristic differences in their mass spectra. These results demonstrate the ability of ToF-SIMS to determine subtle chemical differences even in fixed histological specimens.
ISSN:1387-3806
1873-2798
DOI:10.1016/j.ijms.2006.09.029