Synchrotron-based intravenous cerebral angiography in a small animal model

• Published in: Physics in medicine & biology Vol. 52; no. 4; pp. 1001 - 1012
• Main Authors: Kelly, Michael E, Schültke, Elisabeth, Fiedler, Stephan, Nemoz, Christian, Guzman, Raphael, Corde, Stephanie, Esteve, Francois, LeDuc, Geraldine, Juurlink, Bernhard H J, Meguro, Kotoo
• Format: Journal Article
• Language: English
• Published: England IOP Publishing 21.02.2007
• Subjects: Angiography, Digital Subtraction; Angiography, Digital Subtraction - instrumentation; Angiography, Digital Subtraction - methods; Animals; Cerebral Angiography; Cerebral Angiography - instrumentation; Cerebral Angiography - methods; Cerebral Arteries; Cerebral Arteries - diagnostic imaging; Cerebral Arteries - pathology; Contrast Media; Contrast Media - administration & dosage; Contrast Media - pharmacology; Infusion Pumps; Male; Models, Animal; Rabbits; Radionuclide Imaging; Synchrotrons
• ISSN: 0031-9155; 1361-6560
• DOI: 10.1088/0031-9155/52/4/009

K-edge digital subtraction angiography (KEDSA), a recently developed synchrotron-based technique, utilizes monochromatic radiation and allows acquisition of high-quality angiography images after intravenous administration of contrast agent. We tested KEDSA for its suitability for intravenous cerebral angiography in an animal model. Adult male New Zealand rabbits were subjected to either angiography with conventional x-ray equipment or synchrotron-based intravenous KEDSA, using an iodine-based contrast agent. Angiography with conventional x-ray equipment after intra-arterial administration of contrast agent demonstrated the major intracranial vessels but no smaller branches. KEDSA was able to visualize the major intracranial vessels as well as smaller branches in both radiography mode (planar images) and tomography mode. Visualization was achieved with as little as 0.5 ml kg-1 of iodinated contrast material. We were able to obtain excellent visualization of the cerebral vasculature in an animal model using intravenous injection of contrast material, using synchrotron-based KEDSA.