Hippocampal neuron loss and astrogliosis in medial temporal lobe epileptic patients with mental disorders

• Published in: Journal of integrative neuroscience Vol. 18; no. 2; pp. 127 - 132
• Main Authors: Lu, Jun, Huang, Hongxing, Zeng, Qichang, Zhang, Xinmei, Xu, Min, Cai, Yi, Wang, Qin, Huang, Yahui, Peng, Qiong, Deng, Lanqiuzi
• Format: Journal Article
• Language: English
• Published: England IMR Press 30.06.2019
• Subjects: Adult; Astrocytes - metabolism; Epilepsy, Temporal Lobe - complications; Epilepsy, Temporal Lobe - genetics; Epilepsy, Temporal Lobe - metabolism; Epilepsy, Temporal Lobe - pathology; Female; Gene Expression; Gliosis - genetics; Gliosis - metabolism; Hippocampus - metabolism; Hippocampus - pathology; Humans; Male; medial temporal lobe epilepsy|mental disorder|astrocytes|glial fibrillary acidic protein|metallothionein-i/ii|aquaporin-4; Mental Disorders - complications; Mental Disorders - genetics; Mental Disorders - metabolism; Mental Disorders - pathology; Middle Aged; Neurons - metabolism; Neurons - pathology; metallothionein-I/II; aquaporin-4; Medial temporal lobe epilepsy; glial fibrillary acidic protein; mental disorder; astrocytes
• ISSN: 0219-6352; 1757-448X; 1757-448X
• DOI: 10.31083/j.jin.2019.02.16

Hippocampal neuron loss and reactive astrogliosis are pathological features of medial temporal lobe epilepsy. Here, the expression of hippocampal astrogliosis-associated genes are studied in subjects with medial temporal lobe epilepsy and mental disorders (such as depression, anxiety and psychiatric comorbidities). The relationship between functional changes in hippocampus astrocytes and concurrent mental disorders are discussed. Nissl staining identified medial temporal lobe epilepsy-induced neuronal loss in the CA1 region of hippocampus. Quantitative real-time polymerase chain reaction and immunofluorescence technology were used to detect hippocampus glial fibrillary acidic protein, metallothionein, and aquaporin-4. The hippocampus area of subjects with medial temporal lobe epilepsy (with or without mental disorders) were smaller than the control group. Hippocampal neuronal loss and astrogliosis were more obvious in groups of medial temporal lobe epileptic patients with mental disorders. Relative protein levels of glial fibrillary acidic protein, metallothionein-I/II, and aquaporin-4 were significantly higher in subjects with medial temporal lobe epilepsy than seen in controls. Medial temporal lobe epileptic patients with mental disorder or depression had elevated metallothionein-I/II protein level when compared to controls and medial temporal lobe epileptic patients without mental disorder. Protein levels of glial fibrillary acidic protein and aquaporin-4 in medial temporal lobe epileptic patients with mental disorders were significantly lower than that in medial temporal lobe epileptic patients with no mental disorder. It is concluded that functional changes in hippocampus astrocytes are associated with mental disorders in medial temporal lobe epileptic patients and the astrogliosis-related genes of glial fibrillary acidic protein, metallothionein-I/II and aquaporin-4, are involved in this process.