Characterization of a US Sheep Scrapie Isolate with Short Incubation Time

Scrapie is a naturally occurring fatal neurodegenerative disease of sheep and goats. Susceptibility to the disease is partly dependent upon the genetic makeup of the host. In a previous study it was shown that sheep intracerebrally inoculated with US scrapie inoculum (No. 13-7) developed terminal di...

Full description

Saved in:
Bibliographic Details
Published inVeterinary pathology Vol. 46; no. 6; pp. 1205 - 1212
Main Authors Hamir, A.N, Richt, J.A, Kunkle, R.A, Greenlee, J.J, Bulgin, M.S, Gregori, L, Rohwer, R.G
Format Journal Article
LanguageEnglish
Published Los Angeles, CA American College of Veterinary Pathologists 01.11.2009
SAGE Publications
Subjects
Online AccessGet full text

Cover

Loading…
More Information
Summary:Scrapie is a naturally occurring fatal neurodegenerative disease of sheep and goats. Susceptibility to the disease is partly dependent upon the genetic makeup of the host. In a previous study it was shown that sheep intracerebrally inoculated with US scrapie inoculum (No. 13-7) developed terminal disease within an average of 19 months. We have since produced an inoculum, No. x124 from pooled brains of US-origin sheep scrapie, that results in incubations nearly threefold shorter. The present study documents clinicopathologic findings and the distribution of abnormal prion proteins (PrPSc) by immunohistochemical (IHC) and Western blot (WB) techniques, in tissues of sheep inoculated with No. x124. All inoculated sheep developed clinical disease and were euthanatized within an average of 7.7 months postinoculation (MPI). Sheep that had valine/valine or alamine/valine at codon 136 of prion protein (PRNP) gene developed the disease faster and were euthanatized at an average of 4.3 and 5.6 MPI, respectively. Also, the inoculum was able to induce disease in a short time (7 MPI) in a sheep that was relatively resistant (QR at codon 171) to scrapie. This indicates that inoculum No. x124 appears to induce scrapie in shorter time than inoculum No. 13-7, especially in sheep homozygous or heterozygous for valine at codon 136.
Bibliography:http://hdl.handle.net/10113/37401
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0300-9858
1544-2217
DOI:10.1354/vp.08-VP-0258-H-FL