Biological Imaging with Medium‐Sensitive Bichromatic Flexible Fluorescent Dyes

A family of environment‐sensitive shape‐shifting molecules have been developed as flexible fluorescent (FlexFluor) dyes for biological imaging applications. These compounds feature a flexible bithiophene‐based fluorophore that gives rise to different emission colors in lipophilic or hydrophilic envi...

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Published inAngewandte Chemie International Edition Vol. 56; no. 49; pp. 15603 - 15606
Main Authors Cao, Yang, Wang, Xiaozhu, Shi, Xiaolei, Clee, Susanne M., McGeer, Patrick L., Wolf, Michael O., Orvig, Chris
Format Journal Article
LanguageEnglish
Published WEINHEIM Wiley 04.12.2017
Wiley Subscription Services, Inc
EditionInternational ed. in English
Subjects
adipocytes
bichromatic dyes
Brain
Chemistry
Chemistry, Multidisciplinary
Dyes
Emission
Fluorescence
Fluorescence microscopy
Fluorescent dyes
Fluorescent indicators
Lipophilic
lipophilicity
Marine environment
myelin sheath
Neuroimaging
neurons
Physical Sciences
Science & Technology
Tissues
BODIPY
PROTEIN
SPECTROSCOPIC PROPERTIES
MEMBRANE
MITOCHONDRIA
neurons
MYELIN
bichromatic dyes
MULTIPLE-SCLEROSIS
DISEASE
lipophilicity
myelin sheath
adipocytes
DERIVATIVES
LIPID DROPLETS
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Summary:A family of environment‐sensitive shape‐shifting molecules have been developed as flexible fluorescent (FlexFluor) dyes for biological imaging applications. These compounds feature a flexible bithiophene‐based fluorophore that gives rise to different emission colors in lipophilic or hydrophilic environments, as well as side groups that can be synthetically modified with ease. FlexFluor dyes are the first fluorescent dyes in which emission color can be used to indicate lipid/water environments. The behavior of these dyes in different solvents was studied, and used to simultaneously highlight lipid and water contents in adipose and brain tissues using optical fluorescence microscopy. Dye Another Day: Environment‐sensitive shape‐shifting molecules function as flexible fluorescent (FlexFluor) dyes for biological imaging applications. The color of light emitted from FlexFluor dyes is dependent on the lipid/water content of the environment. Optical fluorescence microscopy can be used to detect simultaneous emissions in adipose and brain tissues containing lipid and water.
Bibliography:These authors contributed equally to this work.
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ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201708529