Multiple‐Color Platinum Complex with Super‐Large Stokes Shift for Super‐Resolution Imaging of Autolysosome Escape

• Published in: Angewandte Chemie International Edition Vol. 59; no. 43; pp. 19229 - 19236
• Main Authors: Liu, Liu‐Yi, Fang, Hongbao, Chen, Qixin, Chan, Michael Ho‐Yeung, Ng, Maggie, Wang, Kang‐Nan, Liu, Wenting, Tian, Zhiqi, Diao, Jiajie, Mao, Zong‐Wan, Yam, Vivian Wing‐Wah
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 19.10.2020
• Edition: International ed. in English
• Subjects: A549 Cells; autolysosome; Biological Transport; Cell Nucleus - metabolism; Color; Coordination compounds; Drug delivery; Drug development; Drugs; HeLa Cells; Humans; Imaging; Lysosomes - metabolism; Metal complexes; Microscopy, Fluorescence - methods; Mitochondria - metabolism; Photobleaching; Platinum; platinum complexes; Platinum Compounds - chemistry; Platinum Compounds - metabolism; real-time tracking; Selectivity; Stokes shift; super-resolution imaging; real-time tracking; platinum complexes; Stokes shift; super-resolution imaging; autolysosome
• ISSN: 1433-7851; 1521-3773; 1521-3773
• DOI: 10.1002/anie.202007878

It is of great significance to track the platinum drugs in real time with super‐resolution to elucidate their mechanism of action, such as their behavior and distribution in live cells. Such information is required for further drug development. However, it is always challenging to design platinum complexes suitable for such research. Herein, we design a luminescent building block (L) for metal complexes and a dinuclear platinum complex (Pt2L) for super‐resolution imaging. Because of its super‐large Stokes shift and excellent photophysical properties, Pt2L is capable of serving as an ideal candidate for super‐resolution imaging with extremely low luminescence background and high photobleaching resistance. Moreover, upon light stimulation, a matter flux of Pt2L escaping from autolysosomes to nucleus was observed, which represents a new transportation path. Utilizing the photoactivated escape properties, we can regulate the nuclear accessibility of Pt2L form autolysosomes with photo‐selectivity, which provides a new way to improve the targeting of platinum drugs. A multiple‐color platinum complex (Pt2L) with super‐large Stokes shift was designed for super‐resolution imaging, showing an extremely low luminescence background and high photobleaching resistance. Moreover, upon light stimulation, a matter flux of Pt2L escaping from autolysosomes to the nucleus is detected, which represents a new transportation path.