An Azo Coupling Strategy for Protein 3‐Nitrotyrosine Derivatization

Herein, a strategy for the selective derivatization of 3‐nitrotyrosine‐containing proteins using the classic azo coupling reaction as the key step is described. This novel approach featured multiple advantages and was successfully applied to detect picomole levels of protein tyrosine nitration in bi...

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Published inChemistry : a European journal Vol. 25; no. 48; pp. 11228 - 11232
Main Authors Liu, Yuxin, Zhou, Pengcheng, Da, Honghong, Jia, Huiyi, Bai, Feifei, Hu, Guodong, Zhang, Baoxin, Fang, Jianguo
Format Journal Article
LanguageEnglish
Published WEINHEIM Wiley 27.08.2019
Wiley Subscription Services, Inc
Subjects
3-nitrotyrosine
azo coupling
Biological properties
Biological samples
bioorthogonal reactions
Chemistry
Chemistry, Multidisciplinary
Coupling
fluorescence
Nitration
Nitrotyrosine
Physical Sciences
protein modifications
Proteins
Science & Technology
Tyrosine
protein modifications
QUANTIFICATION
ANILINE
IDENTIFICATION
MASS-SPECTROMETRY
bioorthogonal reactions
PEPTIDES
fluorescence
TYROSINE
ENRICHMENT
azo coupling
3-nitrotyrosine
LINKER
NITRATION
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Summary:Herein, a strategy for the selective derivatization of 3‐nitrotyrosine‐containing proteins using the classic azo coupling reaction as the key step is described. This novel approach featured multiple advantages and was successfully applied to detect picomole levels of protein tyrosine nitration in biological samples. Fluorescence: An azo coupling strategy for the selective derivatization of 3‐NT‐containing proteins is presented. This strategy allows the specific introduction of a bioorthogonal group to 3‐NT for the detection and enrichment of 3‐NT‐containing proteins, which facilitates the study of the functions of PTN under physiological and pathological conditions.
Bibliography:These authors contributed equally to this work.
ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:0947-6539
1521-3765
DOI:10.1002/chem.201901828