Synergistic Effects of Beta Tri‐Calcium Phosphate and Porcine‐Derived Decellularized Bone Extracellular Matrix in 3D‐Printed Polycaprolactone Scaffold on Bone Regeneration

• Published in: Macromolecular bioscience Vol. 18; no. 6; pp. e1800025 - n/a
• Main Authors: Kim, Jun‐Young, Ahn, Geunseon, Kim, Changhwan, Lee, Jeong‐Seok, Lee, In‐Gyu, An, Sang‐Hyun, Yun, Won‐Soo, Kim, Shin‐Yoon, Shim, Jin‐Hyung
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 01.06.2018
• Subjects: 3D printing; Animals; beta‐tricalcium phosphate; Biocompatibility; Biomedical materials; Bone grafts; Bone growth; Bone matrix; Bone Matrix - chemistry; Bone Regeneration; Calcium; Calcium phosphates; Calcium Phosphates - chemistry; Cell Line; decellularized extracellular matrix; Differentiation (biology); Extracellular matrix; Grafting; Hydrogels; Mechanical properties; Mice; Osteoblasts - cytology; Osteoblasts - metabolism; Polycaprolactone; Polyesters - chemistry; Printing, Three-Dimensional; Regeneration; Regeneration (physiology); scaffold; Scaffolds; Skin & tissue grafts; Substitute bone; Swine; Synergistic effect; Three dimensional printing; Tissue Scaffolds - chemistry; Tricalcium phosphate; beta-tricalcium phosphate; decellularized extracellular matrix; bone regeneration; scaffold; 3D printing
• ISSN: 1616-5187; 1616-5195
• DOI: 10.1002/mabi.201800025

Bone‐derived extracellular matrix (ECM) is widely used in studies on bone regeneration because of its ability to provide a microenvironment of native bone tissue. However, a hydrogel, which is a main type of ECM application, is limited to use for bone graft substitutes due to relative lack of mechanical properties. The present study aims to fabricate a scaffold for guiding effective bone regeneration. A polycaprolactone (PCL)/beta‐tricalcium phosphate (β‐TCP)/bone decellularized extracellular matrix (dECM) scaffold capable of providing physical and physiological environment are fabricated using 3D printing technology and decoration method. PCL/β‐TCP/bone dECM scaffolds exhibit excellent cell seeding efficiency, proliferation, and early and late osteogenic differentiation capacity in vitro. In addition, outstanding results of bone regeneration are observed in PCL/β‐TCP/bone dECM scaffold group in the rabbit calvarial defect model in vivo. These results indicate that PCL/β‐TCP/bone dECM scaffolds have an outstanding potential as bone graft substitutes for effective bone regeneration. Bone‐derived extracellular matrix (bone dECM) extracted from porcine bone tissue and 3D‐printed scaffolds are used for preparation of polycaprolactone/beta‐tricalcium phosphate/bone dECM scaffolds capable of providing a mechanical and physiological environment. The scaffolds display excellent cell proliferation and osteogenic differentiation in vitro and bone formation in vivo. Consequently, the scaffold can be used as a suitable bone graft substitute.