Self‐Assembly of Copper–DNAzyme Nanohybrids for Dual‐Catalytic Tumor Therapy

Despite the great efforts of using DNAzyme for gene therapy, its clinical success is limited by the lack of simple delivery systems and limited anticancer efficacy. Here, we develop a simple approach for the synthesis of hybrid nanostructures that exclusively consist of DNAzyme and Cu2+ with ultra‐h...

Full description

Saved in:
Bibliographic Details
Published inAngewandte Chemie International Edition Vol. 60; no. 26; pp. 14324 - 14328
Main Authors Liu, Congzhi, Chen, Yaoxuan, Zhao, Jian, Wang, Yong, Shao, Yulei, Gu, Zhanjun, Li, Lele, Zhao, Yuliang
Format Journal Article
LanguageEnglish
Published Germany Wiley Subscription Services, Inc 21.06.2021
EditionInternational ed. in English
Subjects
Anticancer properties
Antitumor activity
Cancer therapies
chemodynamic therapy
Copper
Cytotoxicity
DNAzyme
Fenton-like reaction
Free radicals
Gene silencing
Gene therapy
Glutathione
Hydrogen peroxide
Hydroxyl radicals
mRNA
nanohybrid
Toxicity
DNAzyme
nanohybrid
Fenton-like reaction
gene therapy
chemodynamic therapy
Online AccessGet full text

Cover

More Information
Summary:Despite the great efforts of using DNAzyme for gene therapy, its clinical success is limited by the lack of simple delivery systems and limited anticancer efficacy. Here, we develop a simple approach for the synthesis of hybrid nanostructures that exclusively consist of DNAzyme and Cu2+ with ultra‐high loading capacity. The Cu–DNAzyme nanohybrids allow to effectively co‐deliver DNAzyme and Cu2+ into cancer cells for combinational catalytic therapy. The released Cu2+ can be reduced to Cu+ by glutathione and then catalyze endogenous H2O2 to form cytotoxic hydroxyl radicals for chemodynamic therapy (CDT), while the 10–23 DNAzyme enables the catalytic cleavage of VEGFR2 mRNA and activates gene silencing for gene therapy. We demonstrate that the system can efficiently accumulate in the tumor and exhibit amplified cascade antitumor effects with negligible systemic toxicity. Our work paves an extremely simple way to integrate DNAzyme with CDT for the dual‐catalytic tumor treatment. A nanohybrid was reported for highly efficient combined gene silencing and chemodynamic therapy. The system can effectively co‐deliver DNAzyme and Cu2+ into cancer cells for dual‐catalytic therapy to fight against tumors, thus providing an intelligent self‐driven strategy for smart theranostics.
Bibliography:These authors contributed equally to this work.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.202101744