A quest for indigenous truffle helper prokaryotes
Summary Tuber aestivum is the most common European truffle with significant commercial exploitation. Its production originates from natural habitats and from artificially inoculated host tree plantations. Formation of Tuber ectomycorrhizae in host seedling roots is often inefficient. One possible re...
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| Published in | Environmental microbiology reports Vol. 5; no. 3; pp. 346 - 352 |
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| Main Authors | , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
Blackwell Publishing Ltd
01.06.2013
John Wiley & Sons, Inc |
| Subjects | |
| Online Access | Get full text |
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| Summary: | Summary
Tuber aestivum is the most common European truffle with significant commercial exploitation. Its production originates from natural habitats and from artificially inoculated host tree plantations. Formation of Tuber ectomycorrhizae in host seedling roots is often inefficient. One possible reason is the lack of indigenous associative microbes. Here we aimed at metagenetic characterization and cultivation of indigenous prokaryotes associated with T. aestivum in a field transect cutting through the fungus colony margin. Several operational taxonomic units (OTUs) showed close association with the T. aestivum in the ectomycorrhizae and in the soil, but there was no overlap between the associative prokaryotes in the two different habitats. Among those positively associated with the ectomycorrhizae, we identified several bacterial genera belonging to Pseudonocardineae. Extensive isolation efforts yielded many cultures of ectomycorrhizae‐associative bacteria belonging to Rhizobiales and Streptomycineae, but none belonging to the Pseudonocardineae. The specific unculturable Tuber‐associated prokaryotes are likely to play important roles in the biology of these ectomycorrhizal fungi, including modulation of competition with other symbiotic and saprotrophic microbes, facilitation of root penetration and/or accessing mineral nutrients in the soil. However, the ultimate proof of this hypothesis will require isolation of the microbes for metabolic studies, using novel cultivation approaches. |
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| Bibliography: | ark:/67375/WNG-TH9J7SV5-M ArticleID:EMI412014 Czech Science Foundation - No. P504/10/0382; No. RVO61388971; No. RVO67985831 Fig. S1. Principal component analysis showing relationship between the presence of Tuber aestivum in the soil and selected soil properties across the field transect. Arrows with similar orientation represent the scores of parameters with positive correlation.Fig. S2. Abundance of four ectomycorrhizae-associated actinobacterial OTUs (probably members of the suborder Pseudonocardineae) in samples subjected to metagenetic analysis. Gray columns indicate the abundance of the individual OTUs, whereas the black columns indicate the presence of Tuber aestivum in the respective samples, as revealed by PCR with taxon-specific primers.Table S1. Abundances of the different prokaryotic genera (given as % of all sequences assigned to the individual OTU per sample) in the 15 samples of ectomycorrhizae or soil subjected to metagenetic analysis. Detection of Tuber aestivum in the individual samples by PCR with taxon-specific primers is provided above the respective tables.Table S2. Redundancy analysis and Monte Carlo permutation test of the relationship between the presence of Tuber aestivum as explanatory parameter and frequencies of prokaryotic OTUs in ectomycorrhizae and soil samples collected across the T. aestivum colony margin. The result of the analysis of the interaction between the environment type (soil vs. ectomycorrhizae) and T. aestivum as environmental factors is given in the last column. Calcium concentration has been excluded from the analysis due to its colinearity with pH resulting in excessive values of inflation factor. The analysis was carried out in Canoco 4.5 software.Appendix S1. Experimental protocol 1.Appendix S2. Experimental protocol 2.Appendix S3. Experimental protocol 3. istex:AC2A107A70639A0FBECDE9A70E92AC770E83B846 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| ISSN: | 1758-2229 1758-2229 |
| DOI: | 10.1111/1758-2229.12014 |