Solid State Fermentation and production of Rifamycin SV using Amycolatopsis mediterranei

• Published in: Letters in applied microbiology Vol. 60; no. 1; pp. 44 - 51
• Main Authors: Nagavalli, M, Ponamgi, S.P.D, Girijashankar, V, Venkateswar Rao, L
• Format: Journal Article
• Language: English
• Published: England Published for the Society for Applied Bacteriology by Blackwell Scientific Publications [c1985-] 2015; Oxford University Press
• Subjects: Actinomycetales - metabolism; agro‐industrial by‐products; Amycolatopsis mediterranei; Bacteria, Aerobic; Bioreactors; bran; byproducts; cottonseed meal; Culture Media; drugs; Eleusine coracana subsp. coracana; Fermentation; fermentation optimization; Hydrogen-Ion Concentration; inoculum; medicine; mutants; ragi bran; Rifamycin; rifamycins; Rifamycins - biosynthesis; solid state fermentation; strain improvement; submerged fermentation; Temperature; agro-industrial by-products; strain improvement; ragi bran; solid state fermentation; Rifamycin; Amycolatopsis mediterranei; fermentation optimization
• ISSN: 0266-8254; 1472-765X
• DOI: 10.1111/lam.12332

Production of Rifamycin SV from cheaper agro‐industrial by‐products using mutant strain of Amycolatopsis mediterranei OVA5‐E7 in solid state fermentation (SSF) was optimized. Among the agro‐based substrates used, ragi bran was found suitable for maximizing the yield of Rifamycin SV (1310 mg 100 g⁻¹ds). The yield can be further enhanced to 19·7 g Kg⁻¹of dry substrate by supplementing the substrate with deoiled cotton cake (10% w/w) using optimized fermentation parameters such as maintaining 80% moisture, pH 7·0, 30°C incubation temperature, inoculum 25% v/w and carrying the solid state fermenting for 9 days. Manipulating these seven specifications, the end product yield achieved in our experimentation was 20 g of Rifamycin SV Kg⁻¹ds. Eventually, an overall 5‐fold improvement in Rifamycin SV production was achieved. SIGNIFICANCE AND IMPACT OF THE STUDY: Antibiotics such as rifamycin are broad‐spectrum antimicrobial drugs used in large‐scale worldwide as human medicine towards controlling diseases. Amycolatopsis mediterranei strain which produces this antibiotic was earlier used in submerged fermentation yielded lower amounts of rifamycin. By employing cheaper agro‐industrial by‐products, we produced upto 20 g rifamycin SV per Kg dry substrate used under optimized solid state fermentation conditions. Keeping in view, the role of rifamycin in meeting the medical demands of world's increasing population; we successfully used an improved strain on cheaper substrates with optimized fermentation parameters and achieved a 5‐fold improvement in rifamycin SV production.