S-Adenosyl methionine/S-adenosyl-L-homocysteine ratio determination by capillary electrophoresis employed as a monitoring tool for the antiviral effectiveness of adenosine analogs
S‐Adenosyl‐L‐homocysteine hydrolase (SAHh) inhibitors have long been used as broad‐range antivirals and have been recently evaluated as an experimental therapy of filovirus infections. In response to the need for a rapid laboratory testing method that could assess antiviral potency in vivo, our grou...
Saved in:
| Published in | Electrophoresis Vol. 25; no. 10-11; pp. 1518 - 1521 |
|---|---|
| Main Authors | , , , |
| Format | Journal Article |
| Language | English |
| Published |
Weinheim
WILEY-VCH Verlag
01.06.2004
WILEY‐VCH Verlag |
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | S‐Adenosyl‐L‐homocysteine hydrolase (SAHh) inhibitors have long been used as broad‐range antivirals and have been recently evaluated as an experimental therapy of filovirus infections. In response to the need for a rapid laboratory testing method that could assess antiviral potency in vivo, our group developed a capillary electrophoresis (CE) method for the determination of the S‐adenosyl‐L‐homocysteine (SAH) to S‐adenosyl‐L‐methionine (SAM) ratio. After chloroacetaldehyde derivatization, SAH and SAM were detected using laser‐induced fluorescence detection with a HeCd laser. Separation and quantitation of both SAH and SAM in human plasma were achieved in less than 1 min. The proposed method is rapid and reliable, and could be easily applied to routine monitoring of clinical and preclinical trials subjects. |
|---|---|
| AbstractList | S-Adenosyl-L-homocysteine hydrolase (SAHh) inhibitors have long been used as broad-range antivirals and have been recently evaluated as an experimental therapy of filovirus infections. In response to the need for a rapid laboratory testing method that could assess antiviral potency in vivo, our group developed a capillary electrophoresis (CE) method for the determination of the S-adenosyl-L-homocysteine (SAH) to S-adenosyl-L-methionine (SAM) ratio. After chloroacetaldehyde derivatization, SAH and SAM were detected using laser-induced fluorescence detection with a HeCd laser. Separation and quantitation of both SAH and SAM in human plasma were achieved in less than 1 min. The proposed method is rapid and reliable, and could be easily applied to routine monitoring of clinical and preclinical trials subjects.S-Adenosyl-L-homocysteine hydrolase (SAHh) inhibitors have long been used as broad-range antivirals and have been recently evaluated as an experimental therapy of filovirus infections. In response to the need for a rapid laboratory testing method that could assess antiviral potency in vivo, our group developed a capillary electrophoresis (CE) method for the determination of the S-adenosyl-L-homocysteine (SAH) to S-adenosyl-L-methionine (SAM) ratio. After chloroacetaldehyde derivatization, SAH and SAM were detected using laser-induced fluorescence detection with a HeCd laser. Separation and quantitation of both SAH and SAM in human plasma were achieved in less than 1 min. The proposed method is rapid and reliable, and could be easily applied to routine monitoring of clinical and preclinical trials subjects. S-Adenosyl-L-homocysteine hydrolase (SAHh) inhibitors have long been used as broad-range antivirals and have been recently evaluated as an experimental therapy of filovirus infections. In response to the need for a rapid laboratory testing method that could assess antiviral potency in vivo, our group developed a capillary electrophoresis (CE) method for the determination of the S-adenosyl-L-homocysteine (SAH) to S-adenosyl-L-methionine (SAM) ratio. After chloroacetaldehyde derivatization, SAH and SAM were detected using laser-induced fluorescence detection with a HeCd laser. Separation and quantitation of both SAH and SAM in human plasma were achieved in less than 1 min. The proposed method is rapid and reliable, and could be easily applied to routine monitoring of clinical and preclinical trials subjects. S‐Adenosyl‐L‐homocysteine hydrolase (SAHh) inhibitors have long been used as broad‐range antivirals and have been recently evaluated as an experimental therapy of filovirus infections. In response to the need for a rapid laboratory testing method that could assess antiviral potency in vivo, our group developed a capillary electrophoresis (CE) method for the determination of the S‐adenosyl‐L‐homocysteine (SAH) to S‐adenosyl‐L‐methionine (SAM) ratio. After chloroacetaldehyde derivatization, SAH and SAM were detected using laser‐induced fluorescence detection with a HeCd laser. Separation and quantitation of both SAH and SAM in human plasma were achieved in less than 1 min. The proposed method is rapid and reliable, and could be easily applied to routine monitoring of clinical and preclinical trials subjects. S ‐Adenosyl‐ L ‐homocysteine hydrolase (SAHh) inhibitors have long been used as broad‐range antivirals and have been recently evaluated as an experimental therapy of filovirus infections. In response to the need for a rapid laboratory testing method that could assess antiviral potency in vivo , our group developed a capillary electrophoresis (CE) method for the determination of the S ‐adenosyl‐ L ‐homocysteine (SAH) to S ‐adenosyl‐ L ‐methionine (SAM) ratio. After chloroacetaldehyde derivatization, SAH and SAM were detected using laser‐induced fluorescence detection with a HeCd laser. Separation and quantitation of both SAH and SAM in human plasma were achieved in less than 1 min. The proposed method is rapid and reliable, and could be easily applied to routine monitoring of clinical and preclinical trials subjects. S-Adenosyl-L-homocysteine hydrolase (SAHh) inhibitors have long been used as broad-range antivirals and have been recently evaluated as an experimental therapy of filovirus infections. In response to the need for a rapid laboratory testing method thatcould assess antiviral potency in vivo, our group developed a capillary electrophoresis (CE) method for the determination of the S-adenosyl-L-homocysteine (SAH) to S-adenosyl-L-methionine (SAM) ratio. After chloroacetaldehyde derivatization, SAH and SAM were detected using laser-induced fluorescence detection with a HeCd laser. Separation and quantitation of both SAH and SAM in human plasma were achieved in less than 1min. The proposed method is rapid and reliable, and could be easily applied to routine monitoring of clinical and preclinical trials subjects. |
| Author | Sbrana, Elena Spinetti, Maria C. Raspi, Giorgio Bramanti, Emilia |
| Author_xml | – sequence: 1 givenname: Elena surname: Sbrana fullname: Sbrana, Elena email: elsbrana@utmb.edu organization: University Of Texas Medical Branch, Department of Pathology, Galveston, TX, USA – sequence: 2 givenname: Emilia surname: Bramanti fullname: Bramanti, Emilia organization: Laboratory of Instrumental Analytical Chemistry, Institute for Physical Chemistry Processes, National Council of Research, Pisa, Italy – sequence: 3 givenname: Maria C. surname: Spinetti fullname: Spinetti, Maria C. organization: Department of Chemistry, University of Pisa, Pisa, Italy – sequence: 4 givenname: Giorgio surname: Raspi fullname: Raspi, Giorgio organization: Department of Chemistry, University of Pisa, Pisa, Italy |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/15188235$$D View this record in MEDLINE/PubMed |
| BookMark | eNqFksFvFCEUxompsdvq1aPh5G22wAwzzLFp6mqyapPVbOKFMOybLsrACGx1_i7_QVl3rcbE9ESA7_c93vs4QyfOO0DoOSVzSgi7ADvGOSOkJFxw-gjNKGesYLUoT9CM0KYsiCj5KTqL8TMhpGqr6gk6pZwKwUo-Qz9WxeUGnI-TxQOkrfHOOLhYFep4WiyLrR-8nmKCfIODSsbjDSQIg3H7jcPdhLUajbUqTBgs6BT8uPUBookYhtH6CTZYRazwkP2TD8bd4uS9xb0POG0BK5fMnQnKYuj7bGDuwEGM2Pf48JJ9beWU9bfxKXrcKxvh2XE9Rx9fXX-4el0s3y_eXF0uC11RQotaaa15brUXWokOasIaBrykpCI9LwXwuq1BdA2rN0LRTrScK847TaATwJryHL08-I7Bf91BTHIwUUNu04HfRdnkuTd1Ix4U0jZPm5G944ujcNcNsJFjMEOemfydRxbMDwIdfIwB-j8SIveBy33g8j7wDFT_ANqkX6mkoIz9P9YesG_GwvRAEXm9vFn9zRYH1uQv8f2eVeGLrJuy4XL9biHftjdcfFov5Lr8CV3F1G8 |
| CitedBy_id | crossref_primary_10_1016_j_jpba_2018_11_059 crossref_primary_10_1002_pca_1241 crossref_primary_10_1016_j_jpba_2005_01_041 crossref_primary_10_1002_elps_201000292 crossref_primary_10_1002_elps_201400160 crossref_primary_10_1007_s00604_025_07039_7 crossref_primary_10_1002_elps_200500512 crossref_primary_10_3390_molecules28237740 crossref_primary_10_1021_ac102020n crossref_primary_10_1007_s10517_015_3008_4 crossref_primary_10_1002_elps_201600242 crossref_primary_10_1016_j_aca_2021_338233 crossref_primary_10_1002_anie_200703222 crossref_primary_10_1002_ange_200703222 |
| Cites_doi | 10.1086/514316 10.1086/517704 10.1111/j.1432-1033.1986.tb09963.x 10.1006/abio.1994.1453 10.1006/bbrc.1994.2871 10.1016/0006-2952(87)90533-8 10.1016/S0378-4347(97)00213-2 10.1086/514308 10.1016/S0020-2452(97)83915-2 10.1016/0003-2697(86)90036-9 10.1016/S0378-4347(01)00341-3 10.1093/clinids/11.Supplement_4.S750 10.1086/515386 10.1016/S0166-3542(00)00066-8 10.1016/S0731-7085(02)00121-8 10.1016/0006-2952(89)90411-5 10.1053/jinf.1999.0603 10.1016/S0166-3542(02)00018-9 10.1093/clinchem/46.2.265 10.1093/clinchem/46.10.1650 10.1016/S0163-7258(97)00089-2 10.1016/S0021-9673(98)00363-X 10.1016/0003-9861(74)90306-3 |
| ContentType | Journal Article |
| Copyright | Copyright © 2004 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim |
| Copyright_xml | – notice: Copyright © 2004 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim |
| DBID | BSCLL AAYXX CITATION CGR CUY CVF ECM EIF NPM 7QO 7T7 7U9 8FD C1K FR3 H94 P64 7X8 |
| DOI | 10.1002/elps.200305851 |
| DatabaseName | Istex CrossRef Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed Biotechnology Research Abstracts Industrial and Applied Microbiology Abstracts (Microbiology A) Virology and AIDS Abstracts Technology Research Database Environmental Sciences and Pollution Management Engineering Research Database AIDS and Cancer Research Abstracts Biotechnology and BioEngineering Abstracts MEDLINE - Academic |
| DatabaseTitle | CrossRef MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) Virology and AIDS Abstracts Biotechnology Research Abstracts Technology Research Database AIDS and Cancer Research Abstracts Engineering Research Database Industrial and Applied Microbiology Abstracts (Microbiology A) Biotechnology and BioEngineering Abstracts Environmental Sciences and Pollution Management MEDLINE - Academic |
| DatabaseTitleList | MEDLINE - Academic MEDLINE CrossRef Virology and AIDS Abstracts |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Chemistry |
| EISSN | 1522-2683 |
| EndPage | 1521 |
| ExternalDocumentID | 15188235 10_1002_elps_200305851 ELPS200305851 ark_67375_WNG_M9P58ZWG_W |
| Genre | article Journal Article |
| GroupedDBID | --- .3N .GA .GJ .Y3 05W 0R~ 10A 1L6 1OB 1OC 1ZS 31~ 33P 3SF 3WU 4.4 4ZD 50Y 50Z 51W 51X 52M 52N 52O 52P 52S 52T 52U 52W 52X 53G 5GY 5RE 5VS 66C 702 7PT 8-0 8-1 8-3 8-4 8-5 8UM 930 A03 AAESR AAEVG AAHBH AAHHS AANLZ AAONW AASGY AAXRX AAZKR ABCQN ABCUV ABEML ABIJN ABJNI ABPVW ACAHQ ACBWZ ACCFJ ACCZN ACFBH ACGFS ACPOU ACSCC ACXBN ACXQS ADBBV ADEOM ADIZJ ADKYN ADMGS ADOZA ADXAS ADZMN ADZOD AEEZP AEIGN AEIMD AENEX AEQDE AEUQT AEUYR AFBPY AFFNX AFFPM AFGKR AFPWT AFZJQ AHBTC AI. AITYG AIURR AIWBW AJBDE AJXKR ALAGY ALMA_UNASSIGNED_HOLDINGS ALUQN AMBMR AMYDB AQPKS ASPBG ATUGU AUFTA AVWKF AZBYB AZFZN AZVAB BAFTC BDRZF BFHJK BHBCM BMNLL BMXJE BNHUX BROTX BRXPI BSCLL BY8 CS3 D-E D-F DCZOG DPXWK DR1 DR2 DRFUL DRSTM DU5 EBD EBS EJD EMOBN F00 F01 F04 F5P FEDTE G-S G.N GNP GODZA H.T H.X HBH HF~ HGLYW HHY HHZ HVGLF HZ~ IX1 J0M JPC KQQ LATKE LAW LC2 LC3 LEEKS LH4 LH5 LITHE LOXES LP6 LP7 LUTES LW6 LYRES MEWTI MK4 MRFUL MRSTM MSFUL MSSTM MXFUL MXSTM N04 N05 N9A NF~ NNB O66 O9- OIG P2P P2W P2X P4D PALCI Q.N Q11 QB0 QRW R.K RIWAO RJQFR RNS ROL RWI RX1 RYL SAMSI SUPJJ SV3 UB1 V2E VH1 W8V W99 WBKPD WIB WIH WIK WJL WNSPC WOHZO WQJ WRC WRJ WXSBR WYISQ XG1 XPP XV2 Y6R ZGI ZXP ZZTAW ~IA ~KM ~WT AAHQN AAMNL AANHP AAYCA ACRPL ACYXJ ADNMO AFWVQ ALVPJ AAYXX AEYWJ AGHNM AGQPQ AGYGG CITATION CGR CUY CVF ECM EIF NPM 7QO 7T7 7U9 8FD AAMMB AEFGJ AGXDD AIDQK AIDYY C1K FR3 H94 P64 7X8 |
| ID | FETCH-LOGICAL-c4101-6accc5151f8ca8be60272e531040f538e5696e8b726d8a1b8955a55bc0eb8e273 |
| IEDL.DBID | DR2 |
| ISSN | 0173-0835 |
| IngestDate | Fri Jul 11 09:13:18 EDT 2025 Fri Jul 11 10:05:04 EDT 2025 Wed Feb 19 01:41:24 EST 2025 Tue Jul 01 03:04:52 EDT 2025 Thu Apr 24 22:55:20 EDT 2025 Wed Jan 22 16:27:16 EST 2025 Wed Oct 30 09:46:18 EDT 2024 |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 10-11 |
| Language | English |
| License | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c4101-6accc5151f8ca8be60272e531040f538e5696e8b726d8a1b8955a55bc0eb8e273 |
| Notes | istex:A69D420050DADDC80FCB6381C64F2CC12A708368 ark:/67375/WNG-M9P58ZWG-W ArticleID:ELPS200305851 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| PMID | 15188235 |
| PQID | 19882207 |
| PQPubID | 23462 |
| PageCount | 4 |
| ParticipantIDs | proquest_miscellaneous_72007678 proquest_miscellaneous_19882207 pubmed_primary_15188235 crossref_primary_10_1002_elps_200305851 crossref_citationtrail_10_1002_elps_200305851 wiley_primary_10_1002_elps_200305851_ELPS200305851 istex_primary_ark_67375_WNG_M9P58ZWG_W |
| ProviderPackageCode | CITATION AAYXX |
| PublicationCentury | 2000 |
| PublicationDate | 2004-06-01 |
| PublicationDateYYYYMMDD | 2004-06-01 |
| PublicationDate_xml | – month: 06 year: 2004 text: 2004-06-01 day: 01 |
| PublicationDecade | 2000 |
| PublicationPlace | Weinheim |
| PublicationPlace_xml | – name: Weinheim – name: Germany |
| PublicationTitle | Electrophoresis |
| PublicationTitleAlternate | ELECTROPHORESIS |
| PublicationYear | 2004 |
| Publisher | WILEY-VCH Verlag WILEY‐VCH Verlag |
| Publisher_xml | – name: WILEY-VCH Verlag – name: WILEY‐VCH Verlag |
| References | Schnittler, H.-J., Feldmann, H., Clin. Infect. Dis. 1998, 27, 404-406. Chiang, P. K., Pharmacol. Ther. 1998, 77, 115-134. Backlund, P. S., Jr., Carotti, D., Cantoni, G. L., Eur. J. Biochem. 1986, 160, 245-251. Wise, C. K., Cooney, C. A., Ali, S. F., Poirier, L. A., J. Chromatogr. B 1997, 696, 145-152. Finkelstein, J. D., Kyle, W. E., Harris, B. J., Arch. Biochem. Biophys. 1974, 165, 774-779. She, Q. B., Nagao, I., Hayakawa, T., Tsuge, H., Biochem. Biophys. Res. Commun. 1994, 205, 1748-1754. Colebunders, R., Borchert, M., J. Infect. 2000, 40, 16-20. Melnyk, S., Pogribna, M., Pogribny, I. P., Yi, P., James, S. J., Clin. Chem. 2000, 46, 265-272. Castro, R., Struys, E. A., Jansen, E. E. W., Blom, H. J., Tavares de Almeida, I., Jakobs, C., J. Pharm. Biomed. Anal. 2002, 29, 963-968. De Clercq, E., J. Pharm. Exp. Ther. 2001, 297, 1-10. Bray, M., Davis, K., Geisbert, T., Schmaljohn, C., Huggins, J., J. Infect. Dis. 1998, 178, 651-661. Bray, M., Raymond, J. L., Geisbert, T., Baker, R. O., Antiviral Res. 2002, 55, 151-159. Wagner, J., Hirth, Y., Claverie, N., Danzin, C., Anal. Biochem. 1986, 154, 604-617. Wang, W., Kramer, P. M., Yang, S., Pereira, M. A., Tao, L., J. Chromatogr. B 2001, 762, 59-65. Struys, E. A., Jansen, E. E., de Meer, K., Jakobs, C., Clin. Chem. 2000, 46, 1650-1656. Caussé, E., Terrier, R., Champagne, S., Nertz, M., Valdiguié, P., Salvayre, R., Couderc, F., J. Chromatogr. A 1998, 817, 181-185. De Clercq, E., Cools, M., Balzarini, J., Biochem. Pharmacol. 1989, 38, 1771-1778. Bwaka, M. A., Bonnet, M.-J., Calain, P., Colebunders, R., De Roo, A., Guimard, Y., Katwiki, K. R., Kibadi, K., Kipasa, M. A., Kuvula, K. J., Mapanda, B. B., Massamba, M., Mupapa, K. D., Muyembe-Tamfum, J.-J., Ndaberey, E., Peters, C. J., Rollin, P. E., Van den Enden, E., J. Infect. Dis. 1999, 179, S1-S7. De Clercq, E., Biochem. Parmacol. 1987, 36, 2567-2575. Le Guenno, B., Galabru, J., Bull. Inst. Pasteur 1997, 95, 73-83. Huggins, J., Zhang, Z.-X., Bray, M., J. Infect. Dis. 1999, 179, S240-S247. Bray, M., Driscoll, J., Huggins, J. W., Antiviral Res. 2000, 45, 135-147. Huggins, J. W., Rev. Infect. Dis. 1989, 11, S750-S761. Tseng, H. C., Dadoo, R., Zare, R. N., Anal. Biochem. 1994, 222, 55-58. 1998; 27 1997; 696 1994; 222 2001; 297 1987; 36 2001; 762 1989; 11 1997; 95 1994; 205 2002; 29 1986; 154 2000; 45 2000; 46 1986; 160 2002; 55 1998; 817 2000; 40 1999; 179 1989; 38 1974; 165 1998; 178 1998; 77 De Clercq E. (e_1_2_1_2_2) 2001; 297 e_1_2_1_6_2 e_1_2_1_7_2 e_1_2_1_4_2 e_1_2_1_5_2 e_1_2_1_11_2 e_1_2_1_22_2 e_1_2_1_3_2 e_1_2_1_12_2 e_1_2_1_23_2 e_1_2_1_20_2 e_1_2_1_10_2 e_1_2_1_21_2 e_1_2_1_15_2 e_1_2_1_16_2 e_1_2_1_13_2 e_1_2_1_24_2 e_1_2_1_14_2 e_1_2_1_25_2 e_1_2_1_19_2 e_1_2_1_8_2 e_1_2_1_17_2 e_1_2_1_9_2 e_1_2_1_18_2 |
| References_xml | – reference: Chiang, P. K., Pharmacol. Ther. 1998, 77, 115-134. – reference: Huggins, J. W., Rev. Infect. Dis. 1989, 11, S750-S761. – reference: Finkelstein, J. D., Kyle, W. E., Harris, B. J., Arch. Biochem. Biophys. 1974, 165, 774-779. – reference: Melnyk, S., Pogribna, M., Pogribny, I. P., Yi, P., James, S. J., Clin. Chem. 2000, 46, 265-272. – reference: De Clercq, E., Biochem. Parmacol. 1987, 36, 2567-2575. – reference: Le Guenno, B., Galabru, J., Bull. Inst. Pasteur 1997, 95, 73-83. – reference: Bray, M., Davis, K., Geisbert, T., Schmaljohn, C., Huggins, J., J. Infect. Dis. 1998, 178, 651-661. – reference: Wagner, J., Hirth, Y., Claverie, N., Danzin, C., Anal. Biochem. 1986, 154, 604-617. – reference: Bwaka, M. A., Bonnet, M.-J., Calain, P., Colebunders, R., De Roo, A., Guimard, Y., Katwiki, K. R., Kibadi, K., Kipasa, M. A., Kuvula, K. J., Mapanda, B. B., Massamba, M., Mupapa, K. D., Muyembe-Tamfum, J.-J., Ndaberey, E., Peters, C. J., Rollin, P. E., Van den Enden, E., J. Infect. Dis. 1999, 179, S1-S7. – reference: Caussé, E., Terrier, R., Champagne, S., Nertz, M., Valdiguié, P., Salvayre, R., Couderc, F., J. Chromatogr. A 1998, 817, 181-185. – reference: De Clercq, E., Cools, M., Balzarini, J., Biochem. Pharmacol. 1989, 38, 1771-1778. – reference: Bray, M., Driscoll, J., Huggins, J. W., Antiviral Res. 2000, 45, 135-147. – reference: De Clercq, E., J. Pharm. Exp. Ther. 2001, 297, 1-10. – reference: Backlund, P. S., Jr., Carotti, D., Cantoni, G. L., Eur. J. Biochem. 1986, 160, 245-251. – reference: Struys, E. A., Jansen, E. E., de Meer, K., Jakobs, C., Clin. Chem. 2000, 46, 1650-1656. – reference: Castro, R., Struys, E. A., Jansen, E. E. W., Blom, H. J., Tavares de Almeida, I., Jakobs, C., J. Pharm. Biomed. Anal. 2002, 29, 963-968. – reference: Wang, W., Kramer, P. M., Yang, S., Pereira, M. A., Tao, L., J. Chromatogr. B 2001, 762, 59-65. – reference: Wise, C. K., Cooney, C. A., Ali, S. F., Poirier, L. A., J. Chromatogr. B 1997, 696, 145-152. – reference: Bray, M., Raymond, J. L., Geisbert, T., Baker, R. O., Antiviral Res. 2002, 55, 151-159. – reference: She, Q. B., Nagao, I., Hayakawa, T., Tsuge, H., Biochem. Biophys. Res. Commun. 1994, 205, 1748-1754. – reference: Tseng, H. C., Dadoo, R., Zare, R. N., Anal. Biochem. 1994, 222, 55-58. – reference: Schnittler, H.-J., Feldmann, H., Clin. Infect. Dis. 1998, 27, 404-406. – reference: Huggins, J., Zhang, Z.-X., Bray, M., J. Infect. Dis. 1999, 179, S240-S247. – reference: Colebunders, R., Borchert, M., J. Infect. 2000, 40, 16-20. – volume: 95 start-page: 73 year: 1997 end-page: 83 publication-title: Bull. Inst. Pasteur – volume: 165 start-page: 774 year: 1974 end-page: 779 publication-title: Arch. Biochem. Biophys. – volume: 297 start-page: 1 year: 2001 end-page: 10 publication-title: J. Pharm. Exp. Ther. – volume: 27 start-page: 404 year: 1998 end-page: 406 publication-title: Clin. Infect. Dis. – volume: 762 start-page: 59 year: 2001 end-page: 65 publication-title: J. Chromatogr. B – volume: 55 start-page: 151 year: 2002 end-page: 159 publication-title: Antiviral Res. – volume: 160 start-page: 245 year: 1986 end-page: 251 publication-title: Eur. J. Biochem. – volume: 179 start-page: S240 year: 1999 end-page: S247 publication-title: J. Infect. Dis. – volume: 222 start-page: 55 year: 1994 end-page: 58 publication-title: Anal. Biochem. – volume: 77 start-page: 115 year: 1998 end-page: 134 publication-title: Pharmacol. Ther. – volume: 36 start-page: 2567 year: 1987 end-page: 2575 publication-title: Biochem. Parmacol. – volume: 817 start-page: 181 year: 1998 end-page: 185 publication-title: J. Chromatogr. A – volume: 45 start-page: 135 year: 2000 end-page: 147 publication-title: Antiviral Res. – volume: 11 start-page: S750 year: 1989 end-page: S761 publication-title: Rev. Infect. Dis. – volume: 29 start-page: 963 year: 2002 end-page: 968 publication-title: J. Pharm. Biomed. Anal. – volume: 40 start-page: 16 year: 2000 end-page: 20 publication-title: J. Infect. – volume: 205 start-page: 1748 year: 1994 end-page: 1754 publication-title: Biochem. Biophys. Res. Commun. – volume: 46 start-page: 1650 year: 2000 end-page: 1656 publication-title: Clin. Chem. – volume: 46 start-page: 265 year: 2000 end-page: 272 publication-title: Clin. Chem. – volume: 38 start-page: 1771 year: 1989 end-page: 1778 publication-title: Biochem. Pharmacol. – volume: 154 start-page: 604 year: 1986 end-page: 617 publication-title: Anal. Biochem. – volume: 178 start-page: 651 year: 1998 end-page: 661 publication-title: J. Infect. Dis. – volume: 179 start-page: S1 year: 1999 end-page: S7 publication-title: J. Infect. Dis. – volume: 696 start-page: 145 year: 1997 end-page: 152 publication-title: J. Chromatogr. B – ident: e_1_2_1_8_2 doi: 10.1086/514316 – ident: e_1_2_1_14_2 doi: 10.1086/517704 – ident: e_1_2_1_12_2 doi: 10.1111/j.1432-1033.1986.tb09963.x – ident: e_1_2_1_24_2 doi: 10.1006/abio.1994.1453 – ident: e_1_2_1_17_2 doi: 10.1006/bbrc.1994.2871 – ident: e_1_2_1_3_2 doi: 10.1016/0006-2952(87)90533-8 – ident: e_1_2_1_19_2 doi: 10.1016/S0378-4347(97)00213-2 – ident: e_1_2_1_13_2 doi: 10.1086/514308 – ident: e_1_2_1_16_2 doi: 10.1016/S0020-2452(97)83915-2 – ident: e_1_2_1_20_2 doi: 10.1016/0003-2697(86)90036-9 – ident: e_1_2_1_21_2 doi: 10.1016/S0378-4347(01)00341-3 – volume: 297 start-page: 1 year: 2001 ident: e_1_2_1_2_2 publication-title: J. Pharm. Exp. Ther. – ident: e_1_2_1_10_2 doi: 10.1093/clinids/11.Supplement_4.S750 – ident: e_1_2_1_7_2 doi: 10.1086/515386 – ident: e_1_2_1_9_2 doi: 10.1016/S0166-3542(00)00066-8 – ident: e_1_2_1_23_2 doi: 10.1016/S0731-7085(02)00121-8 – ident: e_1_2_1_4_2 doi: 10.1016/0006-2952(89)90411-5 – ident: e_1_2_1_15_2 doi: 10.1053/jinf.1999.0603 – ident: e_1_2_1_11_2 doi: 10.1016/S0166-3542(02)00018-9 – ident: e_1_2_1_18_2 doi: 10.1093/clinchem/46.2.265 – ident: e_1_2_1_22_2 doi: 10.1093/clinchem/46.10.1650 – ident: e_1_2_1_5_2 doi: 10.1016/S0163-7258(97)00089-2 – ident: e_1_2_1_25_2 doi: 10.1016/S0021-9673(98)00363-X – ident: e_1_2_1_6_2 doi: 10.1016/0003-9861(74)90306-3 |
| SSID | ssj0004944 |
| Score | 1.8099843 |
| Snippet | S‐Adenosyl‐L‐homocysteine hydrolase (SAHh) inhibitors have long been used as broad‐range antivirals and have been recently evaluated as an experimental therapy... S ‐Adenosyl‐ L ‐homocysteine hydrolase (SAHh) inhibitors have long been used as broad‐range antivirals and have been recently evaluated as an experimental... S-Adenosyl-L-homocysteine hydrolase (SAHh) inhibitors have long been used as broad-range antivirals and have been recently evaluated as an experimental therapy... |
| SourceID | proquest pubmed crossref wiley istex |
| SourceType | Aggregation Database Index Database Enrichment Source Publisher |
| StartPage | 1518 |
| SubjectTerms | Acetaldehyde - analogs & derivatives Acetaldehyde - chemistry Adenosine analogs Antiviral Agents - pharmacology Antiviral drugs Ebola Electrophoresis, Capillary Filovirus Humans S-Adenosyl-L-homocysteine hydrolase S-Adenosylhomocysteine - analysis S-Adenosylhomocysteine - blood S-Adenosylmethionine - analysis S-Adenosylmethionine - blood |
| Title | S-Adenosyl methionine/S-adenosyl-L-homocysteine ratio determination by capillary electrophoresis employed as a monitoring tool for the antiviral effectiveness of adenosine analogs |
| URI | https://api.istex.fr/ark:/67375/WNG-M9P58ZWG-W/fulltext.pdf https://onlinelibrary.wiley.com/doi/abs/10.1002%2Felps.200305851 https://www.ncbi.nlm.nih.gov/pubmed/15188235 https://www.proquest.com/docview/19882207 https://www.proquest.com/docview/72007678 |
| Volume | 25 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV3LbtQwFLVQWcCG9yM8vUCwSifJxImzrKo-hNqqolRTsbFsx1arpsloMiMxrPgE_oe_4Uu4104yDKJCgkWkScZRPOPjm-Obm3MIecO1NDYzeRhLWKKkJjNhYVkZcg4AkpbZ2JlNHB5l-6fp-zN29stb_F4fYki44cxw8RonuFTtaCUaaqqpk9sGwHL3DjUWbCEr-rDSj0qL1It752OUYWa9amOUjNZPX7sr3cQ_-POfKOc6g3W3oN27RPad95Unl5uLudrUX37TdfyfX3eP3On4Kd3ygLpPbpj6Abm13dvCPSTfT358_bZVosb4sqJoQO0yumaEx2V3HD4ewHbeXDUapaLhe-qgRsu-_AYBQdWSajlF46PZknaOPNPzZmbai5Yab0ZcUtlSSa9c8MEsJJ03TUWBbFMgrxSQcYGFyhX1pSld9KaNpb43eG1ZY46qfUROd3c-bu-HnQNEqFOIFWEmtdbAuGILmOLKZLCITgyEDQg9FkK1YVmRGa7yJCu5jBUvGJOMKR0ZxQ0ws8dko25q85RQBQtZW-SRzSECRcoWOhvHKdNaobl5YQMS9ggQupNHR5eOSnhh50TgkIhhSALybmg_9cIg17Z86wA1NJOzSyyny5mYHO2Jw-KY8U-TPTEJyOsecQIGFZ_cyNo0i1bEBSyEkii_vkWOOWcgHgF54qG66hRK7iVjFpDEAe4vvRU7B8cnw96zfznpObnt65swV_WCbMxnC_MSqNtcvXLT8yft4kP5 |
| linkProvider | Wiley-Blackwell |
| linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV3JbtswECWK5JBeui_qFh6K9qTYkkUtxyBI4ra2ETQJXPRCkBSJBFEkw7KBuqd-Qv-nf9Mv6QwpyXDRoEB7MGDLFEybj6M349F7hLxOldAm1okfCEhRIh1rPzMs99MUACQMM4E1mxhP4uF59P4Ta7sJ8V4Ypw_RFdxwZ9h4jRscC9K9tWqoLmZWbxsQm-JN1Nto622zqo9rBakoi5y8dzJAIWbW6jb2w97m-RvXpW38ib_8iXRuclh7ETq6S2Q7fdd7crW3XMg99fU3Zcf_-n73yJ2GotJ9h6n75JYuH5Cdg9YZ7iH5cfrz2_f9HGXGVwVFD2pb1NU9PC6a4_B0BI-L6rpSqBYN71OLNpq3HTiICSpXVIkZeh_NV7Qx5ZldVHNdX9ZUOz_inIqaCnpt4w8WIumiqgoKfJsCf6UAjkvsVS6o605pAjitDHWzwc8WJZap6kfk_Ojw7GDoNyYQvoogXPixUEoB6QoMwCqVOoY8OtQQOSD6GIjWmsVZrFOZhHGeikCmGWOCMan6WqYayNljslVWpX5KqIRc1mRJ3yQQhPrSZCoeBBFTSqK_eWY84rcQ4KpRSEejjoI7beeQ45Lwbkk88rYbP3PaIDeOfGMR1Q0T8yvsqEsYn06O-Tg7Yenn6TGfemS3hRyHRcU_b0Spq2XNgwxyobCf3DwiwbIzcA-PPHFYXU8KVffCAfNIaBH3l9nyw9HJaffq2b-ctEt2hmfjER-9m3x4Tm67dicsXb0gW4v5Ur8EJreQr-xe_QXxN0gU |
| linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1db9MwFLXQJgEvfDPC1_yA4ClrksaJ8zht6wZ0VcWYOvFi2Y6tTeuaqGklyhM_gf_Dv-GXcG-ctBQxIcFDpCZ1FLc-vjm-uTmHkFdcS2MTk_qhhCVKbBLjZ5blPucAIGmZDWuzieNBcnQavztjZ7-8xe_0IZYJN5wZdbzGCV7mtrMSDTXjspbbBsByfId6M04Cjrje_7ASkIqz2Kl7p13UYWatbGMQddbPX7stbeI__PlPnHOdwtb3oN5dItveu9KTy535TO3oL78JO_7Pz7tH7jQEle46RN0nN8zkAbm11_rCPSTfT358_babo8j4YkzRgbpO6ZoOHpfNcfjYh-28uCo0akXD97TGGs3b-htEBFULqmWJzkfTBW0secrzYmqqi4oa50acU1lRSa_q6INpSDorijEFtk2BvVKAxgVWKo-pq01pwjctLHW9wWvLCSapqkfktHfwce_IbywgfB1DsPATqbUGyhVaABVXJoFVdGQgbkDssRCrDUuyxHCVRknOZah4xphkTOnAKG6Amj0mG5NiYp4QqmAla7M0sCmEoEDZTCfdMGZaK3Q3z6xH_BYBQjf66GjTMRZO2TkSOCRiOSQeebNsXzplkGtbvq4BtWwmp5dYT5cyMRociuNsyPin0aEYeWS7RZyAQcVHN3JiinklwgxWQlGQXt8ixaQzMA-PbDmorjqFmntRl3kkqgH3l96Kg_7wZLn39F9O2iY3h_s90X87eP-M3Ha1Tpi3ek42ZtO5eQE0bqZe1jP1JxJSRsw |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=S-Adenosyl+methionine%2FS-adenosyl-L-homocysteine+ratio+determination+by+capillary+electrophoresis+employed+as+a+monitoring+tool+for+the+antiviral+effectiveness+of+adenosine+analogs&rft.jtitle=Electrophoresis&rft.au=Sbrana%2C+Elena&rft.au=Bramanti%2C+Emilia&rft.au=Spinetti%2C+Maria+C&rft.au=Raspi%2C+Giorgio&rft.date=2004-06-01&rft.issn=0173-0835&rft.volume=25&rft.issue=10-11&rft.spage=1518&rft_id=info:doi/10.1002%2Felps.200305851&rft_id=info%3Apmid%2F15188235&rft.externalDocID=15188235 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0173-0835&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0173-0835&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0173-0835&client=summon |