S-Adenosyl methionine/S-adenosyl-L-homocysteine ratio determination by capillary electrophoresis employed as a monitoring tool for the antiviral effectiveness of adenosine analogs

• Published in: Electrophoresis Vol. 25; no. 10-11; pp. 1518 - 1521
• Main Authors: Sbrana, Elena, Bramanti, Emilia, Spinetti, Maria C., Raspi, Giorgio
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 01.06.2004; WILEY‐VCH Verlag
• Subjects: Acetaldehyde - analogs & derivatives; Acetaldehyde - chemistry; Adenosine analogs; Antiviral Agents - pharmacology; Antiviral drugs; Ebola; Electrophoresis, Capillary; Filovirus; Humans; S-Adenosyl-L-homocysteine hydrolase; S-Adenosylhomocysteine - analysis; S-Adenosylhomocysteine - blood; S-Adenosylmethionine - analysis; S-Adenosylmethionine - blood
• ISSN: 0173-0835; 1522-2683
• DOI: 10.1002/elps.200305851

S‐Adenosyl‐L‐homocysteine hydrolase (SAHh) inhibitors have long been used as broad‐range antivirals and have been recently evaluated as an experimental therapy of filovirus infections. In response to the need for a rapid laboratory testing method that could assess antiviral potency in vivo, our group developed a capillary electrophoresis (CE) method for the determination of the S‐adenosyl‐L‐homocysteine (SAH) to S‐adenosyl‐L‐methionine (SAM) ratio. After chloroacetaldehyde derivatization, SAH and SAM were detected using laser‐induced fluorescence detection with a HeCd laser. Separation and quantitation of both SAH and SAM in human plasma were achieved in less than 1 min. The proposed method is rapid and reliable, and could be easily applied to routine monitoring of clinical and preclinical trials subjects.