Sterol glycosides and cerebrosides accumulate in Pichia pastoris, Rhynchosporium secalis and other fungi under normal conditions or under heat shock and ethanol stress

• Published in: Yeast (Chichester, England) Vol. 18; no. 8; pp. 679 - 695
• Main Authors: Sakaki, Takeshi, Zähringer, Ulrich, Warnecke, Dirk C., Fahl, Annette, Knogge, Wolfgang, Heinz, Ernst
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.06.2001
• Subjects: Acremonium chrysogenum; Candida albicans; Cerebrosides; Cerebrosides - analysis; Cerebrosides - metabolism; Culture Media; dimorphism; Ethanol; Fatty Acids - analysis; Fungi - genetics; Fungi - metabolism; Fungi - physiology; glycolipid; Glycolipids - analysis; Glycolipids - metabolism; Glycosides - analysis; Glycosides - metabolism; Glycosphingolipids - analysis; Hot Temperature; Magnetic Resonance Spectroscopy; Mass Spectrometry; Molecular Sequence Data; pathogenicity; Penicillium olsonii; phospholipids; Phospholipids - analysis; Pichia - metabolism; Pichia - physiology; Pichia anomala; Pichia pastoris; Pyrenophora teres; Rhynchosporium secalis; Sordaria macrospora; Sterol glycosides; Sterols - analysis; steryl glucoside; Triglycerides - analysis; Ustilago maydis
• ISSN: 0749-503X; 1097-0061
• DOI: 10.1002/yea.720

The occurrence of glycolipids such as sterol glycosides, acylated sterol glycosides, cerebrosides and glycosyldiacylglycerols was examined in the three yeast species Candida albicans, Pichia pastoris and Pichia anomala, as well as in the six fungal species Sordaria macrospora, Pyrenophora teres, Ustilago maydis, Acremonium chrysogenum, Penicillium olsonii and Rhynchosporium secalis. Cerebroside was found in all organisms tested, whereas acylated sterol glycosides and glycosyldiacylglycerols were not found in any organism. Sterol glycosides were detected in P. pastoris strain GS115, U. maydis, S. macrospora and R. secalis. This glycolipid occurred in both yeast and filamentous forms of U. maydis but in neither form of C. albicans. This suggests that sterol glycoside is not correlated with the separately grown dimorphic forms of these organisms. Cerebrosides and sterol glycosides from P. pastoris and R. secalis were purified and characterized by mass spectrometry and nuclear magnetic resonance spectroscopy. The cerebrosides are β‐glucosyl ceramides consisting of a saturated α‐hydroxy or non‐hydroxy fatty acid and a Δ4,8‐diunsaturated, C9‐methyl‐branched sphingobase. Sterol glycoside from P. pastoris was identified as ergosterol‐β‐D‐glucopyranoside, whereas the sterol glucosides from R. secalis contain two derivatives of ergosterol. The biosynthesis of sterol glucoside in P. pastoris CBS7435 and GS115 depended on the culture conditions. The amount of sterol glucoside in cells grown in complete medium was much lower than in cells from minimal medium and a strong increase in the content of sterol glucoside was observed when cells were subjected to stress conditions such as heat shock or increased ethanol concentrations. From these data we suggest that, in addition to Saccharomyces cerevisiae, new yeast and fungal model organisms should be used to study the physiological functions of glycolipids in eukaryotic cells. This suggestion is based on the ubiquitous and frequent occurrence of cerebrosides and sterol glycosides, both of which are rarely detected in S. cerevisiae. We suggest P. pastoris and two plant pathogenic fungi to be selected for this approach. Copyright © 2001 John Wiley & Sons, Ltd.