L-glutamic acid-g-poly hydroxyethyl methacrylate nanoparticles: acute and sub-acute toxicity and biodistribution potential in mice

• Published in: Arhiv za higijenu rada i toksikologiju Vol. 74; no. 3; pp. 207 - 217
• Main Authors: Bakan, Buket, Oltulu, Fatih, Yıldırım, Yeliz, Yavaşoğlu, Altuğ, Akgöl, Sinan, Karabay Yavaşoğlu, Nefise Ülkü
• Format: Journal Article
• Language: English
• Published: Zagreb Sciendo 01.09.2023; Institute for Medical Research and Occupational Health
• Subjects: Acute toxicity; Albinism; Biochemistry; Biocompatibility; Biodistribution; biokemija krvi; biokompatibilnost; Blood; blood biochemistry; Bone marrow; Drug delivery; Drug delivery systems; Drug dosages; genotoksičnost; genotoxicity; Glutamic acid; Hematology; histologija; histology; In vivo methods and tests; in vivo toksičnost; in vivo toxicity; Ketamine; Leukocytes; micronucleus test; mikronukleus test; Nanoparticles; Organ weight; Original; polimeri; Polyhydroxyethyl methacrylate; polymers; Statistical analysis; toksičnost; Toxicity; Variance analysis; United States > US; Japan; Turkey
• ISSN: 1848-6312; 0004-1254; 1848-6312
• DOI: 10.2478/aiht-2023-74-3768

The aim of this safety study in mice was to determine toxicity and biodistribution potential of a single and multiple doses of L-glutamic acid-g-p(HEMA) polymeric nanoparticles as a drug delivery system. The single dose did not cause any lethal effect, and its acute oral LD was >2.000 mg/kg body weight (bw). Multiple doses (25, 50, or 100 mg/kg bw) given over 28 days resulted in no significant differences in body and relative organ weights compared to control. These results are supported by biochemical and histological findings. Moreover, nanoparticle exposure did not result in statistically significant differences in micronucleus counts in bone marrow cells compared to control. Nanoparticle distribution was time-dependent, and they reached the organs and even bone marrow by hour 6, as established by imaging with the IVIS spectrum imaging system. In conclusion, L-glutamic acid-g-p(HEMA) polymeric nanoparticles appear biocompatible and have a potential use as a drug delivery system.