Simultaneous Electrochemical Determination of Ascorbic Acid, Dopamine and Uric Acid Based on Reduced Graphene Oxide-Ag/PANI Modified Glassy Carbon Electrode

• Published in: Chemical research in Chinese universities Vol. 33; no. 3; pp. 507 - 512
• Main Authors: Guo, Zhuo, Luo, Xianke, Li, Yahui, Li, Dongdi, Zhao, Qinai, Li, Mengmeng, Ma, Chi, Zhao, Yanting
• Format: Journal Article
• Language: English
• Published: Changchun Jilin University and The Editorial Department of Chemical Research in Chinese Universities 01.06.2017; Springer Nature B.V
• Subjects: Analytical Chemistry; Ascorbic acid; Buffer solutions; Catalysis; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Dopamine; Electrodes; Glassy carbon; Graphene; Inorganic Chemistry; Organic Chemistry; Oxidation; Physical Chemistry; Polyanilines; Reproducibility; Selectivity; Uric acid; Vitamin C; 修饰玻碳电极; 同时测定; 多巴胺; 尿酸; 抗坏血酸; 氧化石墨; 聚苯胺; 还原; Dopamine; Ascorbic acid; Uric acid; Electrochemical measurement
• ISSN: 1005-9040; 2210-3171
• DOI: 10.1007/s40242-017-6473-7

An electrochemical method for simultaneous determination of ascorbic acid（AA）, dopamine（DA） and uric acid（UA） was developed based on RGO-Ag/PANI modified glassy carbon electrode（RGO-Ag/PANI/GCE, RGO= reduced graphene oxide, PANI=polyaniline）. Owing to the synergetic effect among RGO, Ag and PANI, the RGO-Ag/PANI based sensor not only improved the electrochemically catalytic oxidation ofAA, DA and UA, but also resolved the overlapping anodic peaks. Under the optimized conditions, the calibration curves were obtained in the concentration ranges of 50--1000, 5--200 and 20--350 ttmol/L, and the detection limits（S/N=3） of 0.5, 0.2 and 0.2 ~tmol/L were obtained for AA, DA, and UA, respectively, in neutral phosphate buffer solutions（PBS）. The RGO-Ag/PANI/GCE sensor was successfully applied to the determination of AA, DA, and UA in bovine serum samples and showed high selectivity, sensitivity, and reproducibility.