Heparin inhibits mitogen-activated protein kinase activation in intact rat vascular smooth muscle cells
Heparin is potently antiproliferative for vascular smooth muscle cells in vivo and in vitro, inhibiting early proto-oncogene expression and blocking proliferation in the G1 phase of the cell cycle. The mitogen-activated protein kinase (MAPK) family of serine- and threonine-specific kinases is activa...
Saved in:
Published in | The Journal of biological chemistry Vol. 268; no. 26; pp. 19173 - 19176 |
---|---|
Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Bethesda, MD
American Society for Biochemistry and Molecular Biology
15.09.1993
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Heparin is potently antiproliferative for vascular smooth muscle cells in vivo and in vitro, inhibiting early proto-oncogene
expression and blocking proliferation in the G1 phase of the cell cycle. The mitogen-activated protein kinase (MAPK) family
of serine- and threonine-specific kinases is activated in response to a wide range of mitogenic and other factors and is a
key intermediate in cell signaling. We found that heparin inhibits activation of MAPK in response to fetal calf serum and
phorbol 12-myristate 13-acetate, but not epidermal growth factor, revealing heparin-sensitive and -insensitive pathways of
MAPK activation. This report tentatively links suppression of early proto-oncogene expression and inhibition of cellular proliferation
by heparin with inhibition of a mitogenically relevant kinase in living cells. |
---|---|
ISSN: | 0021-9258 1083-351X |
DOI: | 10.1016/S0021-9258(19)36492-0 |