Low temperature stress on the hematological parameters and HSP gene expression in the turbot Scophthalmus maximus

• Published in: Chinese journal of oceanology and limnology Vol. 34; no. 3; pp. 430 - 440
• Main Author: 纪利芹 蒋克勇 刘梅 王宝杰 韩龙江 张明明 王雷
• Format: Journal Article
• Language: English
• Published: Heidelberg Science Press 01.05.2016; Springer Nature B.V
• Subjects: Alanine; Alanine transaminase; Aquaculture; Biological stress; Biology; blood; Blood cells; blood glucose; blood sampling; Cell culture; Cholesterol; cold stress; cooling; cortisol; Creatine; Creatine kinase; Creatinine; Culture tanks; Earth and Environmental Science; Earth Sciences; Enzymatic activity; Enzyme activity; erythrocyte count; Erythrocytes; Fish; Gene expression; Haemoglobin; Heat shock proteins; Hematology; Hemoglobin; Hormones; HSP70; Hsp70 protein; Hsp90 protein; Kinases; L-Lactate dehydrogenase; Lactate; Lactate dehydrogenase; leukocyte count; Leukocytes; Low temperature; Marine; Marine fishes; messenger RNA; Oceanography; Parameters; Polyculture (aquaculture); P基因; Scophthalmus maximus; Serum; Stress; stress response; Studies; tanks; Temperature; Tissue; tissues; Transaminases; triacylglycerols; Triglycerides; turbot; Urea; urea nitrogen; Uric acid; Water temperature; γ-Glutamyltransferase; 乳酸脱氢酶活性; 低温胁迫; 大菱鲆; 血液学参数; 血红蛋白浓度; 鱼体; low temperature stress; hematology; fish; mRNA expression
• ISSN: 0254-4059; 2096-5508; 1993-5005; 2523-3521
• DOI: 10.1007/s00343-016-4367-z

To study the effect of low temperature stress on hematological parameters and HSP gene expression in the turbot （Seophthalmus maximus）, water temperature was lowered rapidly from 18 to 1℃. During the cooling process, three individuals were removed from culture tanks at 18, 13, 8, 5, 3, and 1℃. Blood samples and tissues were taken from each individual, hematological indices and HSP gene expression in tissues were measured. The red blood cell count, white blood cell count, and hemoglobin concentration decreased significantly （P〈0.05） as temperature decreased. Enzyme activities of plasma alanine transaminase and creatine kinase increased as temperature decreased, whereas aspartic transaminase and γ-glutamyl transpeptidase activities displayed no obvious changes above 1℃ and lactate dehydrogenase activity increased first and then decreased. Blood urea nitrogen and uric acid levels were highest at 8℃, and creatinine concentration was highest at 3℃. The concentrations of plasma cortisol, cholesterol, and triglyceride all increased significantly （P〈0.05） as temperature decreased. The serum glucose concentration increased first and then decreased to the initial level. The HSP70 mRNA expression showed various patterns in different tissues, whereas HSP90 mRNA expression showed the same tendency in all tissues. Overall, these results indicate that temperature decreases in the range of 8 to 5℃ may induce a stress response in S. maximus and that temperature should be kept above 8℃ in the aquaculture setting to avoid damage to the fish.