In situ generated thrombin in the protein corona of zeolites： Relevance of the functional proteins to its biological impact

• Published in: Nano research Vol. 7; no. 10; pp. 1457 - 1465
• Main Authors: Li, Yunlong, Liao, Xiaofeng, Zhang, Xiaoxi, Ma, Guicen, Zuo, Shuai, Xiao, Liping, Stucky, Galen D., Wang, Zhugang, Chen, Xian, Shang, Xiaoqiang, Fan, Jie
• Format: Journal Article
• Language: English
• Published: Heidelberg Tsinghua University Press 01.10.2014; Springer Nature B.V
• Subjects: Atomic/Molecular Structure and Spectra; Biochemistry; Biological; Biomedicine; Biotechnology; Cations; Chemistry and Materials Science; Condensed Matter Physics; Coronas; Deactivation; Materials Science; Nanomaterials; Nanostructure; Nanotechnology; Physiology; Plasma; Proteins; Research Article; Surface chemistry; Thrombin; Zeolites; 促凝活性; 功能蛋白; 原位生成; 抗凝血酶; 材料表面; 沸石分子筛; 生物学特性; 钙依赖性; United States > US; China; zeolite; calcium; procoagulant activity; protein corona; thrombin activity
• ISSN: 1998-0124; 1998-0000
• DOI: 10.1007/s12274-014-0505-0

Adsorption of plasma proteins to nanomaterial surfaces has a great influence on their bio-functionality. However, there is limited understanding of the relationship between the functional proteins in the protein corona and the biological identity of the materials. Here we show that the in situ generated thrombin in the protein corona of a Ca-zeolite surface displays a calcium-dependent, unusually high （-3,000 NIH U/mg） procoagulant activity, which is even stable against antithrombin deactivation. Removing the encapsulated Ca^2＋ in the zeolites leads to deactivation by antithrombin. Our observations suggest that the thrombin activity can be regulated by the inorganic surface and cations. Most importantly, our discovery indicates the link between the biomolecules in the protein corona and the procoagulant activity of the materials, providing a new molecular basis for the procoagulant mechanism for zeolite hemostatics.