MicroRNA profiling analysis revealed different cellular senescence mechanisms in human mesenchymal stem cells derived from different origin

Mesenchymal stem cells (MSCs) from human umbilical cord (UC) and cord blood (CB) share many common properties and exhibit promising clinical potential. Cellular senescence, which induces the loss of stem cells characters and disrupts their therapeutic functions, has been demonstrated to be under the...

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Published inGenomics (San Diego, Calif.) Vol. 109; no. 3-4; pp. 147 - 157
Main Authors Meng, Xianhui, Xue, Mengying, Xu, Peng, Hu, Feihu, Sun, Bo, Xiao, Zhongdang
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.07.2017
Subjects
Cellular Senescence
Cord blood
Fetal Blood - cytology
Fetal Blood - physiology
Humans
Mesenchymal stem cell
Mesenchymal Stromal Cells - metabolism
Mesenchymal Stromal Cells - physiology
MicroRNA
MicroRNAs - genetics
Senescence
Transcriptome
Umbilical cord
Umbilical Cord - cytology
Umbilical Cord - physiology
Mesenchymal stem cell
Senescence
MicroRNA
Umbilical cord
Cord blood
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Summary:Mesenchymal stem cells (MSCs) from human umbilical cord (UC) and cord blood (CB) share many common properties and exhibit promising clinical potential. Cellular senescence, which induces the loss of stem cells characters and disrupts their therapeutic functions, has been demonstrated to be under the regulation of microRNAs (miRNAs). In this study, we compared the miRNA profiles in early and late passage UCMSCs and CBMSCs based on deep sequencing. 224 and 170 miRNAs were significantly altered in UCMSCs and CBMSCs respectively. A functional annotation of the predicted miRNA targets revealed a series of common senescence pathways. However, Functional enrichment analysis revealed different bioprocesses involved in cellular senescence of UC- and CB-MSCs. The common miRNAs shared by the two kinds of MSCs also exert different function in terms of GO enrichment analysis. Our results supported MSCs derived from different origin may undergo senescence through different path. •Senescence-related microRNAs were identified and compared in human UCMSCs and CBMSCs.•The effect of microRNAs on senescence of MSCs was evaluated.•MicroRNAs triggered different senescence-related path in UCMSCs and CBMSCs.
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ISSN:0888-7543
1089-8646
DOI:10.1016/j.ygeno.2017.02.003