Anti-inflammatory Effect of Peroxisome Proliferator Activated Receptor Gamma on Human Dental Pulp Cells

• Published in: Journal of endodontics Vol. 35; no. 4; pp. 524 - 528
• Main Authors: Yu, Mi-Kyung, DDS, PhD, Lee, Jung-Chang, PhD, Kim, Jeong-Hee, DDS, PhD, Lee, Young-Hee, PhD, Jeon, Jae-Gyu, DDS, PhD, Jhee, Eun-Chung, PhD, Yi, Ho-Keun, PhD
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.04.2009
• Subjects: Anilides - pharmacology; Anti-Inflammatory Agents - pharmacology; Blotting, Western; Cells, Cultured; Dental Pulp - cytology; Dental Pulp - metabolism; Dentistry; Endocrinology & Metabolism; Escherichia coli - chemistry; Fibroblasts - metabolism; Human dental pulp cells; Humans; ICAM-1; Inflammation Mediators - metabolism; Intercellular Adhesion Molecule-1 - biosynthesis; Lipopolysaccharides; Matrix Metalloproteinase 2 - biosynthesis; Matrix Metalloproteinase 9 - biosynthesis; Matrix Metalloproteinase Inhibitors; MMP-2; MMP-9; PPAR gamma; PPAR gamma - agonists; PPAR gamma - antagonists & inhibitors; PPAR gamma - pharmacology; PPAR gamma - physiology; pulpal inflammation; Pulpitis - metabolism; Recombinant Proteins - pharmacology; Thiazolidinediones - pharmacology; Vascular Cell Adhesion Molecule-1 - biosynthesis; VCAM-1; VCAM-1; Human dental pulp cells; MMP-2; ICAM-1; pulpal inflammation; MMP-9; PPAR gamma
• ISSN: 0099-2399; 1878-3554
• DOI: 10.1016/j.joen.2008.12.012

Abstract Peroxisome proliferator activated receptor gamma (PPARγ) plays a critical role in controlling immune and inflammatory responses. However, its effect on pulpal inflammation has not been clarified. The purpose of this study was to determine the anti-inflammatory effect of PPARγ on pulpal inflammation. Human dental pulp cells treated with lipopolysaccharide exhibited elevated levels of matrix metalloproteinase-2 (MMP-2), MMP-9, intracellular adhesion molecule-1 (ICAM-1), and vascular cell adhesion molecule-1 (VCAM-1). However, when treated with rosiglitazone (PPARγ agonist) or adenoviral PPARγ in same culture system, the expression of ICAM-1 and VCAM-1 was markedly inhibited along with decreased secretion of MMPs. In addition, the coadministration of GW9662 (PPARγ antagonist) and rosiglitazone blocked the inhibition of MMP-2, MMP-9, ICAM-1, and VCAM-1. These results suggest that PPARγ decreased the production of MMPs, ICAM-1, and VCAM-1 and might offer a possible attempt of using it as one of anti-inflammatory modulators in a pulpal inflammation.