Allosteric regulation of glycogen synthase in liver. A physiological dilemma

• Published in: The Journal of biological chemistry Vol. 268; no. 18; pp. 13286 - 13290
• Main Authors: NUTTALL, F. Q, GANNON, M. C
• Format: Journal Article
• Language: English
• Published: Bethesda, MD American Society for Biochemistry and Molecular Biology 25.06.1993
• Subjects: Adenosine - pharmacology; Allosteric Regulation; Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Enzymes and enzyme inhibitors; Fundamental and applied biological sciences. Psychology; Glucose-6-Phosphate; Glucosephosphates - metabolism; Glycogen Synthase - metabolism; Liver - enzymology; Male; Miscellaneous; Nucleotides - pharmacology; Rats; Rats, Sprague-Dawley; Uridine Diphosphate Glucose - metabolism; Vertebrata; Mammalia; Enzymatic activity; Rat; Allosteric regulation; Enzyme; Liver; Rodentia; Kinetics; Glucose
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1016/S0021-9258(19)38650-8

Glycogen synthase catalyzes the transfer of the glucosyl moiety from UDP-glucose to the terminal branch of the glycogen molecule and is considered to be the rate-limiting enzyme for glycogen synthesis. However, under ideal assay conditions, i.e. 37 degrees C with saturating concentrations of UDP-glucose and the activator, glucose-6-P, the maximal catalytic activity of glycogen synthase was only 78% of the in vivo glycogen synthetic rate. Using concentrations of UDP-glucose and glucose-6-P likely to be present in vivo, the rate was only approximately 30%. This prompted us to reassess a possible role of allosteric effectors on synthase activity. Glycogen synthase was assayed at 37 degrees C using dilute, pH 7.0, buffered extracts, initial rate conditions, and UDP-glucose and glucose-6-P concentrations, which approximate those calculated to be present in total liver cell water. Several allosteric effectors were tested. Magnesium and AMP had little effect on activity. Pi, ADP, ATP, and UTP inhibited activity. When a combination of effectors were added at concentrations approximating those present in cell water, synthase activity could account for only 2% of the glycogen synthetic rate. Thus, although allosteric effectors are likely to be playing a major role in regulating synthase enzymic activity in liver cells, to date, a metabolite that can stimulate activity and/or overcome nucleotide inhibition has yet to be identified. If such a metabolite cannot be identified, an additional or alternative pathway for glycogen synthesis must be considered.