Optofluidic Intracavity Spectroscopy of Canine Hemangiosarcoma

• Published in: IEEE transactions on biomedical engineering Vol. 58; no. 4; pp. 853 - 860
• Main Authors: Wang, Weina, Kisker, David W., Thamm, Douglas H., Shao, Hua, Lear, Kevin L.
• Format: Journal Article
• Language: English
• Published: New York, NY IEEE 01.04.2011; Institute of Electrical and Electronics Engineers; The Institute of Electrical and Electronics Engineers, Inc. (IEEE)
• Subjects: Animals; Biological and medical sciences; Biomedical optical imaging; Biophotonics; Cell Line, Tumor; cell refractometry; Cells (biology); Dogs; Equipment Design; Equipment Failure Analysis; Flow Cytometry - instrumentation; Hemangiosarcoma - pathology; Hematologic and hematopoietic diseases; Investigative techniques, diagnostic techniques (general aspects); Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis; Medical sciences; Microfluidic Analytical Techniques - instrumentation; Miscellaneous. Technology; Nephelometry and Turbidimetry - instrumentation; optical cancer diagnostics; Optical Devices; Optical interferometry; Optical receivers; Optical refraction; Optical resonators; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Refractive index; Refractometry - instrumentation; Reproducibility of Results; ROC Curve; Sensitivity and Specificity; Spectroscopy; Spectrum Analysis - instrumentation; Surface emitting lasers; transmission parameters; Vertical cavity surface emitting lasers; Optical interferometry; Monocyte; Sarcoma; Biophotonics; Exploration; Cardiovascular disease; Malignant hemopathy; Malignant tumor; Angiosarcoma; Lymphoma; Differential diagnostic; Vascular disease; Spectrometry; Lymphoproliferative syndrome; Animal; Refractometry; optical cancer diagnostics; Intracavity; Lymphocyte; cell refractometry; transmission parameters; Cancer; Biomedical engineering
• ISSN: 0018-9294; 1558-2531
• DOI: 10.1109/TBME.2010.2045893

The label-free technique of optofluidic intracavity spectroscopy (OFIS) uses light transmitted through a cellular body in a microfluidic optical resonator to distinguish different types of cells by their optical properties. The OFIS technique has differentiated canine hemangiosarcoma (HSA) cells from monocytes in peripheral blood mononuclear cells based on their distinctive transmission spectra. A single characteristic parameter indicative of strong multi-transverse-mode resonances was determined for each cell by forming a linear combination of the mean and standard deviation of the transmission spectra over one free spectral range, excluding the peaks of passive Fabry-Pérot cavities without cells. The difference in the characteristic parameters of HSA and monocyte samples was statistically highly significant with a p-value as low as 10 6. The same method shows that the characteristic parameters of canine lymphoma and lymphocytes are distinct with p <; 0.005. A receiver operating-characteristic curve constructed from t-distributions fit to the HSA and monocyte data indicates that 95% sensitivity and 98% specificity can be simultaneously achieved.