Evaluation and improvement of phosphate solubilization by an isolated bacterium Pantoea agglomerans ZB

• Published in: World journal of microbiology & biotechnology Vol. 36; no. 2; p. 27
• Main Authors: Li, Lingling, Chen, Rongbin, Zuo, Zhenyu, Lv, Zaosheng, Yang, Zhonghua, Mao, Wei, Liu, Yaoning, Zhou, Yuxi, Huang, Jiaqing, Song, Zibing
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.02.2020; Springer Nature B.V
• Subjects: Acetic acid; Agricultural land; Alkaline phosphatase; Aluminum phosphate; Applied Microbiology; Araucaria - microbiology; Bacteria; Biochemistry; Biofertilizers; Biomedical and Life Sciences; Biotechnology; Calcium phosphates; Carbon - chemistry; Carbon sources; Cell culture; Cultivation; Environmental Engineering/Biotechnology; Fermentation; Glucose - chemistry; Hydroxyapatite; Indoleacetic acid; Inoculation; Iron phosphates; Life Sciences; Microbiology; Mushrooms; Nitrogen Fixation; Nitrogenation; Optimization; Organic acids; Original Paper; Pantoea - isolation & purification; Pantoea - metabolism; Pantoea agglomerans; Phosphates; Phosphates - chemistry; Phytase; Planktonic cells; Plant growth; Rhizosphere; Rock phosphate; Semisolids; Soil Microbiology; Solubility; Solubilization; Substrates; Tricalcium phosphate; Biofertilizer; Inoculation; Pre-incubation; Symplasma; Phosphate solubilization; Spent mushroom substrate; Pantoea agglomerans
• ISSN: 0959-3993; 1573-0972
• DOI: 10.1007/s11274-019-2744-4

A phosphate solubilizing bacterium ZB was isolated from the rhizosphere soil of Araucaria , which falls into the species Pantoea agglomerans . Optimization for phosphate solubilization by strain ZB was performed. At optimum culture conditions, the isolate showed great ability of solubilizing different insoluble inorganic phosphate sources viz. Ca 3 (PO 4 ) 2 (TCP), Hydroxyapatite (HP), CaHPO 4 , AlPO 4 , FePO 4 along with rock phosphates (RPs). Inoculation with planktonic cells was found to enhance dissolved phosphorous as compared to that achieved by symplasma inoculation. Besides inoculation with different status of cells, pre-incubation could also exert a great effect on phosphate solubilization ability of P. agglomerans . When isolate ZB was cultured with glucose as carbon sources, phosphorous was more efficiently dissolved from HP and RP without pre-incubation in comparison to that obtained with pre-cultivation. Pre-cultivation, however, was more suitable for P solubilization than no pre-cultivation when bacteria were grown with xylose. A positive correlation was detected between the production of organic acids and phosphate solubilization. P. agglomerans ZB possessed many plant growth promotion traits such as N 2 fixation and production of indole 3-acetic acid, phytase, alkaline phosphatase. Pot experiment showed inoculation with single isolate ZB or biofertilizer prepared from semi-solid fermentation of isolate ZB with spent mushroom substrate (SMS) compost could enhance plant growth with respect to number of leaves, plant leave area, stem diameter, root length, root dry mass, shoot dry mass and biomass when compared to the abiotic control, revealing strain ZB could be a promising environmental-friendly biofertilizer to apply for agricultural field.