Increase in percutaneous muscle biopsy yield with a suction-enhancement technique

• Published in: Journal of applied physiology (1985) Vol. 82; no. 6; pp. 1739 - 1742
• Main Authors: Hennessey, James V, Chromiak, Joseph A, Dellaventura, Shirley, Guertin, Jennifer, Maclean, David B
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Am Physiological Soc 01.06.1997; American Physiological Society
• Subjects: Aged; Animals; Biological and medical sciences; Biopsy, Needle - instrumentation; Biopsy, Needle - methods; Equipment Design; Female; Humans; Investigative techniques, diagnostic techniques (general aspects); Male; Medical sciences; Muscles - pathology; Osteoarticular system. Muscles; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Suction - instrumentation; Swine; Animal model; Vertebrata; Mammalia; Sample size; Biopsy; Animal; Artiodactyla; Technique; Striated muscle; Ungulata; Medical application; Pig
• ISSN: 8750-7587; 1522-1601
• DOI: 10.1152/jappl.1997.82.6.1739

James V. Hennessey, Joseph A. Chromiak, Shirley Dellaventura, Jennifer Guertin, and David B. Maclean Division of Endocrinology, Department of Medicine, Rhode Island Hospital, Providence 02903; and Department of Pathology and Laboratory Medicine, The Miriam Hospital, Brown University School of Medicine, Providence, Rhode Island 02906 Received 13 November 1996; accepted in final form 5 February 1997. Hennessey, James V., Joseph A. Chromiak, Shirley DellaVentura, Jennifer Guertin, and David B. MacLean. Increase in percutaneous muscle biopsy yield with a suction-enhancement technique. J. Appl. Physiol. 82(6): 1739-1742, 1997. The percutaneous muscle biopsy technique is used in clinical practice and biomedical research. We developed a new enhanced-suction technique [suction-enhancing nipples (SEN)] and compared it with techniques currently in practice by assessing biopsy yields on anesthetized pigs. We applied the enhanced-suction technique to human subjects participating in a clinical trial. In the pig, there was a mean 91% (1.9-fold) increase in the size of the samples obtained with the 4-mm needle when SEN was used and a mean 507% (fivefold) increase in sample size when the SEN was applied to the 6-mm needles. Nine passes of the 6-mm needle with SEN obtained from five consecutive human subjects yielded a mean individual sample size of 109.4 mg or 219.4 mg per needle pass when using the double-sample technique. Adequate tissue samples for histomorphometric and other analyses were obtained in all samples obtained. The percutaneous muscle biopsy performed with enhanced suction using inexpensive, readily available nipples enhances tissue yield two- to fivefold. tissue sample size; utility of muscle biopsy procedure; Bergström muscle biopsy needle 0161-7567/97 $5.00 Copyright © 1997 the American Physiological Society