HLA-DQ2-restricted gluten-reactive T cells produce IL-21 but not IL-17 or IL-22

We have analyzed the production of the effector cytokines interleukin (IL)-17, IL-21, and IL-22 in gluten-reactive CD4 + T cells of celiac disease patients, either cultured from small intestinal biopsies or isolated from peripheral blood after an oral gluten challenge. Combining intracellular cytoki...

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Published inMucosal immunology Vol. 3; no. 6; pp. 594 - 601
Main Authors Bodd, M, Ráki, M, Tollefsen, S, Fallang, L E, Bergseng, E, Lundin, K E A, Sollid, L M
Format Journal Article
LanguageEnglish
Published New York Nature Publishing Group US 01.11.2010
Elsevier Limited
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Summary:We have analyzed the production of the effector cytokines interleukin (IL)-17, IL-21, and IL-22 in gluten-reactive CD4 + T cells of celiac disease patients, either cultured from small intestinal biopsies or isolated from peripheral blood after an oral gluten challenge. Combining intracellular cytokine staining with DQ2-α-II gliadin peptide tetramer staining of intestinal polyclonal T-cell lines, we found that gluten-specific T cells produced interferon-γ (IFN-γ) and IL-21, but not IL-17 or IL-22, even if other T cells of the same lines produced these cytokines. Similarly, in DQ2-α-II-specific T cells in peripheral blood of gluten-challenged patients, very few stained for intracellular IL-17, whereas many cells stained for IFN-γ. We conclude that gluten-reactive T cells produce IL-21 and IFN-γ, but not IL-17. Their production of IL-21 suggests a role for this cytokine in the pathogenesis of celiac disease.
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ISSN:1933-0219
1935-3456
DOI:10.1038/mi.2010.36