Serum miR-9 as a prognostic biomarker in patients with osteosarcoma

• Published in: Journal of international medical research Vol. 42; no. 4; pp. 932 - 937
• Main Authors: Fei, Dan, Li, Yang, Zhao, Dongxu, Zhao, Kaijun, Dai, Lu, Gao, Zhongli
• Format: Journal Article
• Language: English
• Published: London, England SAGE Publications 01.08.2014; Sage Publications
• Subjects: Adolescent; Adult; Aged; Biological and medical sciences; Child; Diseases of the osteoarticular system; Female; Genetic Markers - genetics; Humans; Male; Medical sciences; MicroRNAs - blood; MicroRNAs - genetics; Middle Aged; Multiple tumors. Solid tumors. Tumors in childhood (general aspects); Neoplasm Metastasis - genetics; Osteosarcoma - blood; Osteosarcoma - genetics; Osteosarcoma - pathology; Pharmacology. Drug treatments; Prognosis; Retrospective Studies; Reverse Transcriptase Polymerase Chain Reaction; Tumors; Tumors of striated muscle and skeleton; Young Adult; MicroRNA-9; serum; osteosarcoma; Human; Biological fluid; RNA interference; Prognosis; Sarcoma; Diseases of the osteoarticular system; Micro RNA; Biological marker; Malignant tumor; Gene silencing; Osteosarcoma; Serum; Bone; Cancer
• ISSN: 0300-0605; 1473-2300; 1473-2300
• DOI: 10.1177/0300060514534643

Objectives To quantify microRNA-9 (miR-9) concentrations in the serum of patients with osteosarcoma; to explore its relationship with clinicopathological characteristics and prognosis of osteosarcoma. Method Serum miR-9 was quantified via real-time reverse transcription–polymerase chain reaction in patients with osteosarcoma and healthy control subjects. Overall survival was evaluated using the Kaplan–Meier method. Results Serum miR-9 was significantly upregulated in patients with osteosarcoma (n = 118) compared with healthy control subjects (n = 60); its upregulation was significantly associated with advanced tumour–node–metastasis stage, larger tumour size and presence of distant metastasis. Overall survival duration was significantly shorter in patients with relatively high miR-9 concentrations compared with those with relatively low miR-9 concentrations. Conclusions Serum miR-9 concentrations are significantly increased in patients with osteosarcoma compared with healthy controls. Upregulation of miR-9 is associated with tumour stage, size and metastasis. Serum miR-9 quantification may represent a useful diagnostic and prognostic marker of osteosarcoma.