Thermal tolerance evaluation and related microsatellite marker screening and identification in the large yellow croaker Larimichthys crocea

Thermal tolerance to high temperature was evaluated in the large yellow croaker Larimichthys crocea. The survival thermal maximum for L. crocea was 33.0℃, the 50% critical thermal maximum （50% CTMax） was 35.5℃, and the critical thermal maximum （CTMax） was 36.0℃. Three microsatellite markers （LYC0148...

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Published inChinese journal of oceanology and limnology Vol. 35; no. 3; pp. 566 - 571
Main Author 陈小明 李佳凯 王志勇 蔡明夷 刘贤德
Format Journal Article
LanguageEnglish
Published Heidelberg Science Press 01.05.2017
Springer Nature B.V
Subjects
Biology
Biomarkers
Breeding
Earth and Environmental Science
Earth Sciences
Fish
Fragments
Genetic markers
heat tolerance
High temperature
Larimichthys crocea
Marine
Marine ecology
Microsatellites
molecular cloning
Ocean temperature
Oceanography
screening
Segregation
sequence analysis
Survival
Temperature tolerance
Thermal stress
Tolerance
临界温度
微卫星标记
热耐受性
片段长度
筛选
评价
鉴定
黄色
microsatellite markers
thermal tolerance
large yellow croaker
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Summary:Thermal tolerance to high temperature was evaluated in the large yellow croaker Larimichthys crocea. The survival thermal maximum for L. crocea was 33.0℃, the 50% critical thermal maximum （50% CTMax） was 35.5℃, and the critical thermal maximum （CTMax） was 36.0℃. Three microsatellite markers （LYC0148, LYC0200 and LYC0435）, associated with thermal tolerance were screened and identified using a Bulked Segregation Analysis （BSA） method. These markers have six amplified fragments in which four are related to thermal tolerance. These fragments were cloned and sequenced, and the results showed the core motif were all ＂AC＂ repeats. For LYC0148 and LYC0200, the lengths of fragments are 18l bp and 197 bp, respectively. For LYC0435, which has two fragments, the fragment lengths are 112 bp and 100 bp. The results provide useful molecular markers for thermal-tolerance breeding of large yellow croaker in the near future.
Bibliography:large yellow croaker; thermal tolerance; microsatellite markers
CHEN Xiaoming , LI Jiakai , WANG Zhiyong , CAI Mingyi , LIU Xiande （ Key Laboratory of Mariculture for the East China Sea, Ministry of Agriculture; Fisheries College, Jimei University, Xiamen 361021, China）
37-1150/P
Thermal tolerance to high temperature was evaluated in the large yellow croaker Larimichthys crocea. The survival thermal maximum for L. crocea was 33.0℃, the 50% critical thermal maximum （50% CTMax） was 35.5℃, and the critical thermal maximum （CTMax） was 36.0℃. Three microsatellite markers （LYC0148, LYC0200 and LYC0435）, associated with thermal tolerance were screened and identified using a Bulked Segregation Analysis （BSA） method. These markers have six amplified fragments in which four are related to thermal tolerance. These fragments were cloned and sequenced, and the results showed the core motif were all ＂AC＂ repeats. For LYC0148 and LYC0200, the lengths of fragments are 18l bp and 197 bp, respectively. For LYC0435, which has two fragments, the fragment lengths are 112 bp and 100 bp. The results provide useful molecular markers for thermal-tolerance breeding of large yellow croaker in the near future.
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
ISSN:0254-4059
2096-5508
1993-5005
2523-3521
DOI:10.1007/s00343-017-5342-z