Citrate-Capped AuNP Fabrication, Characterization and Comparison with Commercially Produced Nanoparticles

• Published in: Crystals (Basel) Vol. 12; no. 12; p. 1747
• Main Authors: Memon, Abdul, Channa, Iftikhar, Shaikh, Asif, Ahmad, Jabran, Soomro, Abdul, Giwa, Abdulmoseen, Baig, Zenab, Mahdi, Wael, Alshehri, Sultan
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 01.12.2022
• Subjects: Absorbance; Antiinfectives and antibacterials; AuNP; Biosensors; Bouguer law; Colorimetry; Contaminants; Cost control; Dispersion; Face centered cubic lattice; Gold; gold nanoparticle; High resolution electron microscopy; Laws, regulations and rules; Methods; Morphology; Nanoparticles; nanosensors; Optoelectronics; Reagents; Scanning electron microscopy; Spectrum analysis; Synthesis; TEM; Tissue engineering; Transmission electron microscopy; Turkevich method; X-ray diffraction; Zeta potential; Pakistan; United Kingdom > UK; Japan
• ISSN: 2073-4352; 2073-4352
• DOI: 10.3390/cryst12121747

Gold nanoparticles (AuNPs) were synthesized using citrate reduction, also known as the Turkevich method. The AuNPs were compared with the commercially available product and later subjected to characterization. The AuNPs were 13 nm in diameter with a 2.7 × 108 M−1cm−1 extension coefficient. The calculated concentration was 5.1 nM through the Beer–Lambert law using UV–vis absorbance spectra. Further detailed characterization was applied, such as scanning electron microscopy (SEM), high-resolution transmission electron microscopy (HR-TEM), elemental analysis using electro and DLS instruments, energy-dispersive X-ray spectroscopy (EDS), XRD, and Zeta potential. The synthesized AuNPs had a higher UV-absorbance peak of 0.93 in comparison to commercially available nanoparticles at 5.8 identical conditions. The characterization confirmed successful fabrication of colloidal-citrate-capped AuNPs and their dispersed and aggregated state with induced salt concentration. The shape and morphology were confirmed through XRD, showing a face-centered cubic lattice of {111}, confirmed at 38.1 round shape, and a crystalline lattice. AuNPs tend to be applied in sensing, detection, drug delivery, pharmaceuticals, and other applications in the environment and materials. Other applications include environmental contaminant detection, colorimetric sensors, antimicrobial applications, biosensing and drug delivery, tissue engineering, nanomedicines, optoelectronics, and catalysts.