Long Noncoding RNA HIF1A-AS2 Promotes Non-Small Cell Lung Cancer Progression by the miR-153-5p/S100A14 Axis

• Published in: OncoTargets and therapy Vol. 13; pp. 8715 - 8722
• Main Authors: Zhang, Weiqiang, Liu, Keqiang, Pei, Yingxin, Tan, Jian, Ma, Jingbo, Zhao, Jing
• Format: Journal Article
• Language: English
• Published: New Zealand Taylor & Francis Ltd 01.01.2020; Dove
• Subjects: Apoptosis; Biomarkers; Cell adhesion & migration; Cell migration; Cell proliferation; Cholecystokinin; Gene expression; Lung cancer; Medical prognosis; Metastasis; MicroRNAs; Non-small cell lung carcinoma; Original Research; Proteins; Reagents; Ribonucleic acid; RNA; Small cell lung carcinoma; United States > US; China; Japan; HIF1A-AS2; S100A14; NSCLC; miR-153-5p
• ISSN: 1178-6930; 1178-6930
• DOI: 10.2147/OTT.S262293

Long noncoding RNA (lncRNA) plays a critical role in initiating lung cancer. This study aims to research the function and mechanism of lncRNA HIF1A-AS2 in regulating non-small cell lung cancer (NSCLC) progression. qRT-PCR was used to analyze gene expression. The CCK-8 assay was performed to detect cell proliferation. The Transwell assay was conducted to examine cell migration and invasion. A Caspase3 activity detection kit was utilized to analyze apoptosis. The luciferase reporter assay was carried out to research interactions of HIF1A-AS2, miR-153-5p and S100A14. HIF1A-AS2 expression was raised in NSCLC tissues and cell lines. The HIF1A-AS2 level was increased in advanced NSCLC tumor tissues. High HIF1A-AS2 expression was related to poor prognosis. HIF1A-AS2 knockdown decreased proliferation, migration and invasion while promoting apoptosis. HIF1A-AS2 was the sponge for miR-153-5p, and miR-153-5p targeted S100A14. HIF1A-AS2 promoted S100A14 expression through regulating miR-153-5p. The HIF1A-AS2/miR-153-5p/S100A14 axis plays a crucial role in promoting NSCLC progression.