Glucocorticoid receptor in chick erythrocytes

Glucocorticoid receptor in chick erythrocytes has been characterized. The Scatchard plot analysis of (3H)-dexamethasone binding to red cells showed a single class of binding sites. The apparent Kd of (3H)-dexamethasone binding by whole cell assay was 0.33 nM and the maximum binding capacity was 5.1...

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Bibliographic Details
Published inLife sciences (1973) Vol. 33; no. 15; p. 1485
Main Authors Murakami, T, Ohsawa, N, Takaku, F
Format Journal Article
LanguageEnglish
Published Netherlands 10.10.1983
Subjects
Animals
Binding, Competitive
Chickens - blood
Dexamethasone - metabolism
Erythrocytes - metabolism
Leukocytes - metabolism
Male
Receptors, Glucocorticoid - metabolism
Receptors, Steroid - metabolism
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Summary:Glucocorticoid receptor in chick erythrocytes has been characterized. The Scatchard plot analysis of (3H)-dexamethasone binding to red cells showed a single class of binding sites. The apparent Kd of (3H)-dexamethasone binding by whole cell assay was 0.33 nM and the maximum binding capacity was 5.1 fmole/10(7) cells. The apparent Kd of (3H)-dexamethasone binding to cytosol receptor was 0.48 nM. Steroid binding specificity was similar to that reported for leukocytes.
Bibliography:L
L50
ISSN:0024-3205
1879-0631
DOI:10.1016/0024-3205(83)90851-2