Statistical Optimization of 1,3-Propanediol (1,3-PD) Production from Crude Glycerol by Considering Four Objectives: 1,3-PD Concentration, Yield, Selectivity, and Productivity

• Published in: Applied biochemistry and biotechnology Vol. 186; no. 3; pp. 644 - 661
• Main Authors: Supaporn, Pansuwan, Yeom, Sung Ho
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.11.2018; Springer Nature B.V
• Subjects: 1,3-Propanediol; Ammonium sulfate; Biochemistry; Biodiesel fuels; Biofuels; Biotechnology; Biotechnology - methods; Biotechnology - statistics & numerical data; Chemistry; Chemistry and Materials Science; Cultivation; Glycerol; Glycerol - chemistry; Hydrogen-Ion Concentration; Klebsiella pneumoniae - metabolism; Mathematical models; Models, Statistical; Objectives; Optimization; pH effects; Predictions; Productivity; Propylene Glycols - chemical synthesis; Selectivity; Statistical analysis; Statistical models; Trace elements; Biological conversion; Statistical analysis; Crude glycerol; 1,3-propanediol; Optimization
• ISSN: 0273-2289; 1559-0291
• DOI: 10.1007/s12010-018-2766-7

This study investigated the biological conversion of crude glycerol generated from a commercial biodiesel production plant as a by-product to 1,3-propanediol (1,3-PD). Statistical analysis was employed to derive a statistical model for the individual and interactive effects of glycerol, (NH 4 ) 2 SO 4 , trace elements, pH, and cultivation time on the four objectives: 1,3-PD concentration, yield, selectivity, and productivity. Optimum conditions for each objective with its maximum value were predicted by statistical optimization, and experiments under the optimum conditions verified the predictions. In addition, by systematic analysis of the values of four objectives, optimum conditions for 1,3-PD concentration (49.8 g/L initial glycerol, 4.0 g/L of (NH 4 ) 2 SO 4 , 2.0 mL/L of trace element, pH 7.5, and 11.2 h of cultivation time) were determined to be the global optimum culture conditions for 1,3-PD production. Under these conditions, we could achieve high 1,3-PD yield (47.4%), 1,3-PD selectivity (88.8%), and 1,3-PD productivity (2.1/g/L/h) as well as high 1,3-PD concentration (23.6 g/L).