Protective role of 20-hydroxyecdysone against lead stress in Chlorella vulgaris cultures

• Published in: Phytochemistry (Oxford) Vol. 65; no. 6; pp. 711 - 720
• Main Authors: Bajguz, Andrzej, Godlewska-Zylkiewicz, Beata
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Ltd 01.03.2004; Elsevier
• Subjects: 20-Hydroxyecdysone; Alga; Algal Proteins - metabolism; Animal, plant and microbial ecology; Applied ecology; Biological and medical sciences; Carbohydrate Metabolism; Cell Count; Cells, Cultured; Chlorella - cytology; Chlorella - drug effects; Chlorella - physiology; Chlorella vulgaris; Chlorophyll - metabolism; Ecdysterone - pharmacology; Ecotoxicology, biological effects of pollution; Effects of pollution and side effects of pesticides on plants and fungi; Fundamental and applied biological sciences. Psychology; Glutathione; Growth; Lead - metabolism; Lead - toxicity; Lead stress; Metalloproteins - metabolism; Phytochelatins; Plant Diseases - chemically induced; Trebouxiophyceae; Growth; Chlorella vulgaris; Lead stress; Trebouxiophyceae; Alga; 20-Hydroxyecdysone; Phytochelatins; Phytotoxicity; Chlorophyta; Algae; Lead; Chemical composition; Pollutant effect; Chlorophyceae; Metabolism; Heavy metal; Thallophyta
• ISSN: 0031-9422; 1873-3700
• DOI: 10.1016/j.phytochem.2004.01.015

Treatment of cultured C. vulgaris cells with 10 −6–10 −4 M lead decreased their growth and chemical composition during the first 48 h of cultivation. However, at concentrations above 10 −4 M, lead is cytotoxic to Chlorella vulgaris cells, resulting in cellular fragmentation and lysis. In contrast, at concentrations below 10 −6 M lead had no influence on the growth and metabolism of C. vulgaris cells. 20-Hydroxyecdysone (20E) (10 −10–10 −8 M) increased growth and chemical composition of C. vulgaris cells over a concentration range. Levels per cell of chlorophylls, protein, sugars are all increased by 20E treatment, when compared to non-treated control cells. However, the cultures treated with 20E and lead show a lower stimulation than the cultures treated with 20E alone. The effects of 20E mixed with lead on the growth and the level of cellular lead, chlorophyll, sugar and protein in C. vulgaris are also reported. The decreased growth and composition of C. vulgaris cells treated with lead was restored by the 20E. Application of 20E to C. vulgaris cultures reduced the impact of lead stress on growth, prevented chlorophyll, sugar and protein loss and increased phytochelatins synthesis. Furthermore, 20E did not restore toxic effect of lead on C. vulgaris cells. The combined treatment with lead and 20E appeared to have a stimulatory effect on the above parameters during the 48 h of cultivation, as compared to the control. 20E reduced the toxicity of lead and the growth recovered to the level of cells treated with 20E alone. Concentration-dependent stimulation was observed with increasing concentration of 20E and decreasing concentration of lead. Treatment of cultured Chlorella vulgaris cells with 10 −6–10 −4 M lead decreased their growth and chemical composition during the first 48 h of cultivation. The effects of 20-hydroxyecdysone (20E) (10 −10–10 −8 M) alone or mixed with lead on the growth and the level of cellular lead, chlorophyll, sugar and protein in C. vulgaris are also reported. Application of 20E to C. vulgaris cultures reduced the impact of lead stress on growth, prevented chlorophyll, sugar and protein loss and increased phytochelatins synthesis.