Oat beta-glucan and xylan hydrolysates as selective substrates for Bifidobacterium and Lactobacillus strains

• Published in: Applied microbiology and biotechnology Vol. 49; no. 2; pp. 175 - 181
• Main Authors: Jaskari, J, Kontula, P, Siitonen, A, Jousimies-Somer, H, Mattila-Sandholm, T, Poutanen, K
• Format: Journal Article
• Language: English
• Published: Berlin Springer 01.02.1998
• Subjects: Bacteriology; beta-Glucans; Bifidobacterium - growth & development; Bifidobacterium - metabolism; Biological and medical sciences; Biotechnology; Carbohydrate Metabolism; Chromatography, High Pressure Liquid; Fundamental and applied biological sciences. Psychology; Glucans - metabolism; Growth, nutrition, cell differenciation; intestinal microorganisms; Intestinal Mucosa - microbiology; Lactobacillus - growth & development; Lactobacillus - metabolism; Microbiology; Oligosaccharides - metabolism; Oligosaccharides - pharmacology; probiotic bacteria; Probiotics; Raffinose - metabolism; Raffinose - pharmacology; Substrate Specificity; Xylans - metabolism; Probiotic; Microorganism growth; Oat; Bifidobacterium; Actinomycetaceae; Oligosaccharide; Xylan; Stimulation; Fructose; Raffinose; Actinomycetales; Glucan; Hydrolysis; Substrate; Bacteria; Lactobacillaceae; Nutrient; Actinomycetes; Comparative study; Lactobacillus; Enzymatic reaction
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s002530051155

Novel oligomers that resist digestion in the upper gut were prepared from oat mixed-linked beta-glucan and xylan by enzymatic hydrolysis with lichenase of Bacillus subtilis and xylanase of Trichoderma reesei respectively. The low-molecular-mass hydrolysis products of beta-glucan and xylan were compared with fructooligomers and raffinose in their ability to provide growth substrates for probiotic (Lactobacillus and Bifidobacterium) and intestinal (Bacteroides, Clostridium and Escherichia coli) strains in vitro. A degradation profile of each carbohydrate and total sugar consumption were analysed with HPLC, and bacterial growth rate with an automatic turbidometer, the Bioscreen C system beta-Glucooligomers and xylooligomers both enhanced the growth of health-promoting probiotic strains as compared with intestinal bacterial growth, but not to a significant level. Raffinose stimulated the probiotic strains significantly, whereas fructooligomers induced high average growth for intestinal bacteria also.