Vector and Cell Line Engineering Technologies Toward Recombinant Protein Expression in Mammalian Cell Lines

• Published in: Applied biochemistry and biotechnology Vol. 185; no. 4; pp. 986 - 1003
• Main Authors: Jazayeri, Seyedeh Hoda, Amiri-Yekta, Amir, Bahrami, Salahadin, Gourabi, Hamid, Sanati, Mohammad Hossein, Khorramizadeh, Mohammad Reza
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.08.2018; Springer Nature B.V
• Subjects: Biochemistry; Biopharmaceuticals; Biotechnology; Cell culture; Cell lines; Cells; Chemistry; Chemistry and Materials Science; Chromatin; Deoxyribonucleic acid; DNA; Gene expression; Mammals; Protein engineering; Proteins; Tissue engineering; Vector space; Vector engineering; Cell line engineering; Mammalian cell culture; Recombinant protein expression
• ISSN: 0273-2289; 1559-0291
• DOI: 10.1007/s12010-017-2689-8

The rapid growth of global biopharmaceutical market in the recent years has been a good indication of its significance in biotechnology industry. During a long period of time in recombinant protein production from 1980s, optimizations in both upstream and downstream processes were launched. In this regard, one of the most promising strategies is expression vector engineering technology based on incorporation of DNA opening elements found in the chromatin border regions of vectors as well as targeting gene integration. Along with these approaches, cell line engineering has revealed convenient outcomes in isolating high-producing clones. According to the fact that more than 50% of the approved therapeutic proteins is being manufactured in mammalian cell lines, in this review, we focus on several approaches and developments in vector and cell line engineering technologies in mammalian cell culture.