Comparison of two commercial extenders for cryopreservation of goat semen without sperm washing

• Published in: Theriogenology Vol. 77; no. 2; pp. 412 - 420
• Main Authors: Roof, D.J., Bowley, S., Price, L.L., Matsas, D.J.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.01.2012
• Subjects: Animals; Caprine germplasm; cryopreservation; Cryopreservation - methods; Cryopreservation - veterinary; Cryoprotective Agents; Egg Yolk; freezing; Glycine max; Goat semen; Goats; Male; semen; Semen Analysis - methods; Semen Analysis - veterinary; Semen cryopreservation; Semen extender; semen extenders; Semen Preservation - methods; Semen Preservation - veterinary; Sperm Motility; spermatozoa; washing; Semen extender; Goat semen; Semen cryopreservation; Sperm motility; Caprine germplasm
• ISSN: 0093-691X; 1879-3231
• DOI: 10.1016/j.theriogenology.2011.08.015

The objective of this study was to evaluate the effects of two commercially available semen extenders on the motility of cryopreserved goat sperm and to simplify the cryopreservation protocol. Individual goat ejaculates were split and processed in parallel for freezing in either commercially available soy-based extender (Bioxcell®) or egg yolk-based extender (Irvine TYB). Sperm quality was assessed using total and progressive sperm motility, measured by computer-assisted sperm analysis (CASA). Total motility was higher for samples processed in soy-based extender, both at pre-freeze (P = 0.002) and at post-thaw (P < 0.0001). Progressive motility was higher for semen processed in soy extender at post-thaw (P < 0.0001). Approximately 10% of samples processed in egg yolk-based extender had a large (> 50%) reduction in total motility prior to freezing. However, this type of extreme reduction in pre-freeze motility did not occur in semen samples processed in soy extender. In addition, the use of soy-based extender eliminated the need for a time-consuming sperm washing protocol. We concluded that a commercially available soy-based extender was superior to an egg yolk-based extender in preserving motility of cryopreserved goat sperm, using a two-step method.