Effects of Cultural Conditions in Enhancing the Production of Anti-MRSA Activity of Lasiodiplodia pseudotheobromae IBRL OS-64, an Endophytic Fungus Isolated from Leaf of Ocimum sanctum L. in Submerged Fermentation System

The effects of cultural conditions on the production of anti-MRSA activity of the endophytic fungal isolate, Lasiodiplodia pseudotheobromae IBRL OS-64 isolated from the leaf of a local medicinal herb, Ocimum sanctum Linn. was studied. The highest anti-MRSA activity of 50.0[+ or -]0.1 U/mL and 1.46[+...

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Published inJournal of pure & applied microbiology : an international research journal of microbiology Vol. 13; no. 4; pp. 2517 - 2531
Main Authors Taufiq, M.M.J, Darah, I
Format Journal Article
LanguageEnglish
Published Oriental Scientific Publishing Company 01.12.2019
Journal of Pure and Applied Microbiology
Subjects
Analysis
anti-mrsa activity
Biological products
endophytic fungus
Fermentation
Fungi
lasiodiplodia pseudotheobromae ibrl os-64
Medicinal plants
Medicine, Botanic
Medicine, Herbal
Methicillin
ocimum sanctum linn
Operating systems
Staphylococcus aureus
Staphylococcus aureus infections
submerged fermentation
Malaysia
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Summary:The effects of cultural conditions on the production of anti-MRSA activity of the endophytic fungal isolate, Lasiodiplodia pseudotheobromae IBRL OS-64 isolated from the leaf of a local medicinal herb, Ocimum sanctum Linn. was studied. The highest anti-MRSA activity of 50.0[+ or -]0.1 U/mL and 1.46[+ or -]0.1 g/L of fungal growth were obtained after incorporating all the improved cultural conditions which consisted of yeast extract sucrose broth supplemented with host plant extract, with initial medium pH of 6, 2 mycelial plugs of 4 days old seed culture, cultivation temperature of 30[degrees]C and cultivated for 16 days under static conditions without the presence of light. After improvement of cultural conditions, a significant increment of anti-MRSA activity of about 57.57% (2.4 folds) and a slight increment of fungal growth of about 8.15% (1.1 folds) were obtained compared to the cultural condition before improvement. Indeed the improvement of cultural conditions greatly affected the anti-MRSA activity and growth production by L. pseudotheobromae IBRL OS-64 isolate. Keywords: Endophytic fungus, Lasiodiplodia pseudotheobromae IBRL OS-64, Ocimum sanctum Linn, anti-MRSA activity, submerged fermentation.
ISSN:0973-7510
2581-690X
DOI:10.22207/JPAM.13.4.67