Dipeptide nanoparticle and aptamer-based hybrid fluorescence platform for enrofloxacin determination
A novel fluorescence platform was fabricated for enrofloxacin determination by using cDNA-modified dipeptide fluorescence nanoparticles (FDNP-cDNA) and aptamer-modified magnetic Fe 3 O 4 nanoparticles (Fe 3 O 4 -Apt). The FDNP were prepared via tryptophan-phenylalanine self-assembling. When magnetic...
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Published in | Mikrochimica acta (1966) Vol. 189; no. 3; p. 96 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Vienna
Springer Vienna
01.03.2022
Springer Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | A novel fluorescence platform was fabricated for enrofloxacin determination by using cDNA-modified dipeptide fluorescence nanoparticles (FDNP-cDNA) and aptamer-modified magnetic Fe
3
O
4
nanoparticles (Fe
3
O
4
-Apt). The FDNP were prepared via tryptophan-phenylalanine self-assembling. When magnetic Fe
3
O
4
-Apt incubated with standard solution or sample extracts, the target enrofloxacin was selectively captured by the aptamer on the surface of the Fe
3
O
4
nanoparticles. After removing interference by washing with phosphate-buffered saline, the FDNP-cDNA was added, which can bind to the aptamer on the surface of the Fe
3
O
4
nanoparticles not occupied by the analyte. The higher the concentration of the target enrofloxacin in the standard or sample solution is, the less the FDNP-cDNA can be bound with the Fe
3
O
4
nanoparticles, and the more the FDNP-cDNA can be observed in the supernatant. Fluorescence intensity (E
x
/E
m
= 310/380 nm) increased linearly in the enrofloxacin concentration range 0.70 to 10.0 ng/mL with a detection limit of 0.26 ng/mL (S/N = 3). Good recoveries (88.17–99.30%) were obtained in spiked lake water, chicken, and eel samples with relative standard deviation of 2.7–6.2% (
n
= 3).
Graphical abstract |
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ISSN: | 0026-3672 1436-5073 |
DOI: | 10.1007/s00604-022-05182-z |