High-level secretory production of recombinant porcine follicle-stimulating hormone by Pichia pastoris

• Published in: Process biochemistry (1991) Vol. 36; no. 8; pp. 907 - 913
• Main Authors: Hélène, Boze, Céline, Laborde, Patrick, Chemardin, Fabien, Richard, Christine, Venturin, Yves, Combarnous, Guy, Moulin
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 2001; Elsevier
• Subjects: Computer Science; Follicle-stimulating hormone; Heterologous protein; High cell density cultures; Life Sciences; Medium composition; Pichia pastoris; Follicle-stimulating hormone; High cell density cultures; Pichia pastoris; Medium composition; Heterologous protein; BIOLOGIE CELLULAIRE; BIOTECHNOLOGIE; FSH; HORMONE GONADOTROPE
• ISSN: 1359-5113; 1873-3298
• DOI: 10.1016/S0032-9592(00)00296-X

An active porcine Follicle-Stimulating Hormone (rFSH) was secreted by a recombinant strain of the methylotrophic yeast Pichia pastoris. The influence of the carbon source, either methanol or a mixture of methanol with glycerol or sorbitol, on rFSH production was subsequently studied. On a sorbitol/methanol medium using a high cell-density fed-batch culture the rFSH concentration was 93 mg l −1. The addition, in fed-batch culture, of vitamins and trace elements to the synthetic medium allows optimal biomass and recombinant protein production to be attained. Under these conditions, 187 mg l −1 of rFSH were produced and the production yield Y rFSH/X reached 2.5 mg rFSH g DCW −1. In continuous culture the carbon source and growth factor supplementation had an identical effect on rFSH synthesis, which reached a maximum concentration of 280 mg l −1 and a production yield of 3.6 mg rFSH g DCW −1.